Epidemiologic Characteristics and DNA Sequencing of SRSV Causing Gastroenteritis Outbreak in Two Elementary School.
- Author:
Hyun Kyun KI
1
;
Young Hwa JUNG
;
Jae Keun JUNG
;
Jin Jong SEO
;
Sun Hee KIM
;
Hye Young KEE
;
Doo Sung CHUN
;
Youngmee JI
;
Dong Ryong HA
Author Information
1. Department of Health and Hygiene, Gwangju Metropolitan City, Korea. drha57@hanmail.net
- Publication Type:Original Article
- Keywords:
SRSV;
Genogroup;
Viral gastroenteritis;
Outbreak
- MeSH:
Abdominal Pain;
Adenoviridae;
Bacteria;
Diarrhea;
Disease Outbreaks;
DNA*;
DNA-Directed RNA Polymerases;
Enteritis;
Gastroenteritis*;
Genotype;
Headache;
Humans;
Nausea;
Polymerase Chain Reaction;
Pupil;
Salmonella;
Sequence Analysis, DNA*;
Vomiting
- From:
Infection and Chemotherapy
2003;35(2):107-114
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Small round structured virus (SRSV) is one of the common etiologic agents of viral gastroenteritis in childhood. However, SRSV has not easily been detected in many diarrheal outbreaks. Therefore, we experienced simultaneous outbreak of SRSV enteritis in two elementary schools, therefore, we analyzed the result of the surveillance. METHODS: We interviewed the pupil of two schools and questioned their demographic data, presence of gastrointestinal symptoms, contact with symptomatic persons within the last two weeks, and dietary history within the last one week in school. The specimens of the symptomatic pupil were collected for identification of bacteria (Shigella spp, Salmonella spp, E.coli O157 : H7) and viruses (Norwalk agent, SRSV, adenovirus, and astrovirus). RESULTS: The number of symptomatic patients was 193 (193/2843, 6.8%) and the distribution of symptom onset was unipolar. Frequent symptoms of the patients were abdominal pain (176/193, 91.2%), headache (111/193, 57.5%), vomiting (102/193, 52.8%), diarrhea (83/ 193, 43.0%), febrile sense (79/193, 40.9%), nausea (73/193, 37.8%), chilling (49/193, 25.4%), and tenesmus (8/193, 4.1%). We identified SRSV in 9 cases by PCR method, and analyzed the RNA polymerase gene by DNA sequencing; 2 genogroup (Genogroup I, II) and 3 genotype. CONCLUSION: We confirmed SRSV enteritis with different genotypes in two concurrent outbreaks. DNA sequencing and comparison of genotype among the isolated SRSV in near future are be necessary
