Rapid Detection of Extended Spectrum beta-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method.

Author: Junyoung KIM ¹ ; Semi JEON ; Hogeun RHIE ; Bokkwon LEE ; Misun PARK ; Hoanjong LEE ; Jina LEE ; Seonghan KIM
Author Information

1. Division of Enteric Bacterial Infections, Center for Infectious Disease, National Institute of Health, Seoul, Korea. kkingsh@chol.com
Publication Type:Note
Keywords: Extended Spectrum-beta Lactamase (ESBL); Rapid classification; Multiplex PCR
MeSH: Bacterial Proteins; beta-Lactamases; Enterobacteriaceae; Multiplex Polymerase Chain Reaction; Oxytocin; Polymerase Chain Reaction
From: Infection and Chemotherapy 2009;41(3):181-184
CountryRepublic of Korea
Language:English
Abstract: A multiplex PCR method has been developed to classify extended spectrum beta-lactamase (ESBL) and plasmid-mediated AmpC beta-lactamase (PABL). This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHA type beta-lactamases. We have compared findings from the use of conventional detection methods with that of this newly developed typing method. In testing for 73 ESBL-producing and PABL-producing isolates, 100% of the isolates were correctly identified as previously characterized types and, 44 types of beta-lactamases were additionally identified from 33 isolates. This assay not only reduces the time for classification but also increases the accuracy for detection.