Measurement of HIV-1-specific T Cell Immunity by Using Recombinant Gag Protein.
- Author:
Hyun JEONG
1
;
Seong Heon WIE
;
Youn Jeong KIM
;
Sang Il KIM
;
Yang Ree KIM
;
Tai Gyu KIM
;
Se Hwan YANG
;
Young Chul SUNG
;
Moon Won KANG
Author Information
1. Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea. Infect@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
ELISPOT;
HIV;
Iinterferon-gamma;
Recombinant Gag Protein;
Cell-mediated immunity
- MeSH:
Antiretroviral Therapy, Highly Active;
CD4 Lymphocyte Count;
Enzyme-Linked Immunospot Assay;
Gene Products, gag*;
Haplotypes;
HIV;
HIV Infections;
HIV-1;
Humans;
Immunity, Cellular;
Interferon-gamma;
RNA;
T-Lymphocytes, Cytotoxic
- From:
Infection and Chemotherapy
2006;38(6):374-382
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: HIV-specific immunity, such as strong CD4+ helper and CD8+ cytotoxic T lymphocytes (CTL) responses, develops soon after HIV infection, but usually it can not control HIV replication which ultimately results in severe immune deficiency. HIV-specific CTLs, which are induced by HIV-specific CD4+ helper responses, are the key to cellular immune control of HIV. Measurement of HIV-1-specific CTLs using recombinant Gag protein may be very useful, because it is not restricted by HLA haplotype of the infected individual. MATERIALS AND METHODS: Enzyme-linked immunospot (ELISPOT) assays by using recombinant Gag protein were performed to evaluate HIV-1-specific gamma-interferon cellular responses of 25 HIV-1 infected Korean patients, who had been treated at least for the prior 12 months with highly active antiretroviral therapy at Catholic University Kangnam St. Mary's Hospital. RESULTS: The study group consisted of 25 chronically HIV-infected individuals with a median age of 51 years. The median CD4 counts were 556/mm3 (range:369-994/mm3) and HIV RNA titers were < 25 copies/mL (range: <25-180copies/mL). HIV-1-specific ELISPOT assay results range from 0 to 49 SFCs/2 x 10(5) PBMCs (median, 23.5 SFCs/2 x 10(5) PBMCs). CMV pp65-specific ELISPOT assay results range from 5 to 591 SFCs/2 x 10(5) PBMCs (median, 34 SFCs/2 x 10(5) PBMCs). There was no correlation between CD4 counts and HIV-1-specific SFCs measured by ELISPOT using recombinant protein. CONCLUSION: ELISPOT assays by using recombinant Gag protein may be considerable value in the assessment of cell-mediated immunity of HIV-1 infected patients.
