Effect of resveratrol on the metastasis of 4T1 mouse breast cancer cells in vitro and in vivo.
- Author:
Hyun Sook LEE
1
;
Ae Wha HA
;
Woo Kyoung KIM
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: Resveratrol; metastasis; matrix metalloproteinase-9; 4T1 breast cancer cell; BALB/c mice
- MeSH: Animals; Breast; Breast Neoplasms; Cell Adhesion; Matrix Metalloproteinase 9; Mice; Neoplasm Metastasis; Plasma; Stilbenes; Veins
- From:Nutrition Research and Practice 2012;6(4):294-300
- CountryRepublic of Korea
- Language:English
- Abstract: We investigated the effects of resveratrol on metastasis in in vitro and in vivo systems. 4T1 cells were cultured in the presence of various concentrations (0-30 micromol/L) of resveratrol. For experimental metastasis, BALB/c mice were injected intravenously with 4T1 cells in the tail vein, and were orally administered various concentrations (0, 100, or 200 mg/kg Body weight) of resveratrol for 21 days. After resveratrol treatment, cell adhesion, wound migration, invasion, and MMP-9 activity were significantly decreased in a dose-dependent manner in 4T1 cells (P < 0.05). The numbers of pulmonary nodules were significantly decreased in mice fed the resveratrol (P < 0.05). The plasma MMP-9 activity was decreased in response to treatment with resveratrol in mice (P < 0.05). We conclude that resveratrol inhibits cancer metastasis both in vitro and in vivo, and this inhibition is likely due to the decrease in MMP-9 activity caused by resveratrol.
