Immunomodulation Effect of the Allogeneic Cellular Components after Transfusion.
10.5045/kjh.2007.42.4.375
- Author:
Jung Jin KIM
1
;
Hyo Joo YOO
;
Young Joo KWON
;
Nak Gyun CHUNG
;
Bin CHO
;
Tae Kyu KIM
;
Hack Ki KIM
;
Chi Wha HAN
;
Dae Chul JEONG
Author Information
1. Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. dcjeong@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Transfusion;
Immunomodulation;
IL-10;
CD4+CD25+ cells
- MeSH:
Animals;
Blood Transfusion;
Bone Marrow;
Bromodeoxyuridine;
Cell Proliferation;
Cytokines;
Enzyme-Linked Immunosorbent Assay;
Humans;
Immunomodulation*;
Interleukin-10;
Interleukin-2;
Lymphocytes;
Mice;
Mortality;
Recurrence;
Spleen;
Thymus Gland;
Transforming Growth Factor beta;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Hematology
2007;42(4):375-381
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Blood transfusion is important for life saving treatment in many patients with tolerable adverse effects. Some data suggest that transfusions might cause an increased risk for post-operative infections and a higher relapse or mortality rate in cancer patients. We investigated whether immune dysfunction might result after transfusions from the cellular components. METHODS: We studied 5-week-old mice BALB/c (H-2d, donor), C3H/He (H-2k, recipient), and C57/BL (H-2b, third party). We obtained irradiated spleen cells (SP) from the BALB/c or C57/BL, and injected them into the C3H/He with intraperitoneal IL-2 administration. After 24 hours, we obtained bone marrow (BM), thymus and SP. We identified mixed lymphocyte proliferation (MLR) by the BrdU method and we used irradiated BALB/c SP, as a stimulator for that trial. For the analysis of immune cells, we analyzed the cell surface markers from each organ. For cytokines, we identified TNF-alpha, IFN-gamma, TGF-beta, and IL-10 by ELISA from the supernatant of the MLR. RESULTS: The cell proliferation decreased according to specific H-2 complexes. There were increased CD4+CD25+ cells in the thymus. For the paracrine effects, the B-C3H SP showed ratio-dependent inhibitory effects, although the C-C3H SP inhibited some cell proliferation. There was no difference in the IFN-gamma, TNF-alpha and TGF-beta between the control and experimental groups. However, IL-10 was higher in the 1:10 ratio mixture in the control and transfused SP compared to the other groups. CONCLUSION: The results of this study suggested that the cellular components in transfusions might contribute to the immune regulatory effects by CD4+CD25+ cells after 24 hours.
