Ex Vivo Expansion of Cryopreserved Cord Blood Cells.
- Author:
Samyong KIM
1
;
Chul Hee KIM
;
Gwang Bong BAE
;
Hyun Soo KIM
;
Sang Jun PARK
;
Jong Suk KIM
;
Hwan Jung YUN
;
Deog Yeon JO
Author Information
1. Department of Internal Medicine, Chungnam National University College of Medicine, Taejon, Korea.
- Publication Type:Original Article
- Keywords:
Cord blood cells;
Hematopoietic growth factors;
Ex vivo expansion
- MeSH:
Adult;
Bone Marrow;
Child;
Erythroid Precursor Cells;
Erythropoietin;
Fetal Blood*;
Granulocyte Colony-Stimulating Factor;
Granulocyte-Macrophage Colony-Stimulating Factor;
Granulocyte-Macrophage Progenitor Cells;
Granulocytes;
Hematopoietic Stem Cell Transplantation;
Humans;
Intercellular Signaling Peptides and Proteins;
Interleukin-1beta;
Interleukin-3;
Interleukin-6;
Macrophages;
Stem Cell Factor
- From:Korean Journal of Hematology
1997;32(3):347-359
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Cord blood (CB), which has no HLA restriction, is an alternative to bone marrow for hematopoietic stem cell transplantation. The use of cord blood, however, is limited by the number of progenitor/stem cells necessary to reconstitute the older child or adult. Therefore, ex vivo expansion of CB could have tremendous impact on diverse clinical settings. We studied the ex vivo expansion of isolated population of CD34+ cells from cryopreserved CB cells. METHODS: CD34+ cells were isolated from cryopreserved CB mononuclear cells. Purified cells were cultured with various combinations of hematopoietic growth factors including erythropoietin (EPO), stem cell factor (SCF), granulocyte-colony-stimulating factor (G-CSF), granulocyte, macrophage-colony-stimulating factor (GM-CSF), interleukin-1beta (IL-1beta), IL-3, and IL-6. After 7, 10 or 14 days of culture, the fold increases of colony-forming unit- granulocyte, macrophage (CFU-GM), burst-forming unit-erythroid (BFU-E), colony-forming unit-mix (CFU-Mix), and high proliferative potential colony-forming cell (HPP-CFC) were evaluated. RESULTS: Ten-day culture with the combination of EPO, SCF, G-CSF, IL-1beta, and IL-3 resulted in a median of 60-fold increase of CFU-GM, which was greater than those with the combinations of less than 5 growth factors. The addition of IL-6 or GM-CSF to this combination did not enhance CFU-GM expansion. Ten-day culture was significantly superior to 7-day culture for CFU-GM expansion. Prolongation of culture to 14 days, however, revealed decreased expansion of CFU-GM compared to 10 days. BFU-E and CFU-Mix were expanded to 2~5 folds in 7-day culture with the combination of EPO, SCF, and G-CSF. Further expansion was not achieved in 10-day culture and colonies disappeared in 14-day culture. HPP-CFC was expanded to a median of 7.5 folds in 7-day culture with the combination of EPO, SCF, G-CSF, IL-1beta, IL-3, and IL-6. Neither 10-day or 14 day-culture enhanced expansion of HPP-CFU. CONCLUSION: Cryopreserved cord blood cells maintain ex vivo expansion potential. In our system, 10-day culture with the combination consisting of EPO, SCF, G-CSF, IL-1beta, and IL-3 seems to be adequate for hematopoietic progenitor/stem cell expansion from cryopreserved cord blood cells.
