Elevated levels of activated and inactivated thrombin-activatable fibrinolysis inhibitor in patients with sepsis.
10.5045/kjh.2010.45.4.264
- Author:
Rojin PARK
1
;
Jaewoo SONG
;
Seong Soo AN
Author Information
1. Department of Laboratory Medicine, Soonchunhyang University Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Sepsis;
TAFI isoforms;
TAFIa;
TAFIai;
Diagnosis
- MeSH:
Carboxypeptidase U;
Enzyme-Linked Immunosorbent Assay;
Fibrinolysin;
Fibrinolysis;
Humans;
Organothiophosphorus Compounds;
Plasma;
Protein Isoforms;
Sepsis;
Solanum tuberosum;
Thrombin
- From:Korean Journal of Hematology
2010;45(4):264-268
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: In sepsis, large scale inflammatory responses can cause extensive collateral damage to the vasculature, because both coagulation and fibrinolysis are activated unevenly. Thrombin-activatable fibrinolysis inhibitor (TAFI) plays a role in modulating fibrinolysis. Since TAFI can be activated by both thrombin and plasmin, it is thought to be affected in sepsis. Hence, activated and inactivated TAFI (TAFIa/ai) may be used to monitor changes in sepsis. METHODS: TAFIa/ai-specific in-house ELISA can detect only the TAFIa/ai form, because the ELISA capture agent is potato tuber carboxypeptidase inhibitor (PTCI), which has selective affinity towards only the TAFIa and TAFIai isoforms. TAFIa/ai levels in plasma from 25 patients with sepsis and 19 healthy volunteers were quantitated with the in-house ELISA. RESULTS: We observed increased TAFIa/ai levels in samples from patients with sepsis (48.7+/-9.3 ng/mL) than in samples from healthy individuals (10.5+/-5.9 ng/mL). In contrast, no difference in total TAFI concentration was obtained between sepsis patients and healthy controls. The results suggest that TAFI zymogen was activated and that TAFIa/ai accumulated in sepsis. CONCLUSION: The detection of TAFIa/ai in plasma could provide a useful and simple diagnostic tool for sepsis. Uneven activation of both coagulation and fibrinolysis in sepsis could be caused by the activation of TAFI zymogen and elevation of TAFIa/ai. TAFIa/ai could be a novel marker to monitor sepsis and other blood-related disturbances.
