Lineage-specific chimerism analysis in nucleated cells, T cells and natural killer cells after myeloablative allogeneic hematopoietic stem cell transplantation.
- Author:
Ri Young GOH
1
;
Sung Hyun KIM
;
Jin Yeong HAN
Author Information
- Publication Type:Original Article
- Keywords: Lineage-specific chimerism; Myeloablative stem cell transplantation; T cells; Natural killer cells
- MeSH: Cell Lineage; Chimerism; DNA; Graft Rejection; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells; Humans; Killer Cells, Natural; Lymphocyte Subsets; Lymphocytes; Recurrence; Stem Cell Transplantation; T-Lymphocytes; Tissue Donors
- From:Korean Journal of Hematology 2011;46(1):18-23
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Chimerism analysis is an important tool for assessing the origin of hematopoietic cells after allogeneic stem cell transplantation (allo-SCT) and can be used to detect impending graft rejection and the recurrence of underlying malignant or nonmalignant diseases. METHODS: This study included 24 patients who underwent myeloablative allo-SCT. DNA was extracted from nucleated cells (NCs), T cells, and natural killer (NK) cells, and the chimerism status of these cell fractions was determined by STR-PCR performed using an automated fluorescent DNA analyzer. RESULTS: Twenty-three out of the 24 patients achieved engraftment. Mixed chimerism (MC) in NCs, but not in T cells and NK cells, was significantly correlated with disease relapse. MC in all cell fractions was correlated with mortality. Ten patients (41.6%) developed extensive chronic GVHD. Six patients had MC in T cells, and 3 of them had chronic GVHD. Four patients with MC and relapse received donor lymphocyte infusion (DLI), and among them, 3 had secondary relapse. Further, the chimerism status differed among different cell lineages in 6 patients with myeloid malignancies. CONCLUSION: The implications of MC in lymphocyte subsets are an important area for future research. Chimerism analysis in lineage-specific cells permits detection of relapse and facilitates the monitoring of therapeutic interventions. These results can provide the basic data for chimerism analysis after myeloablative SCT.
