The Amount of Hematopoietic Stem Cell and the Distribution of HLA in Human Cord Blood: Basic Study for the Development of Cord Blood Bank.
- Author:
Sang We KIM
1
;
Sang Hee KIM
;
Tae Won KIM
;
Shin KIM
;
Jeong Sun PARK
;
Je Hwan LEE
;
Sung Bae KIM
;
Cheolwon SUH
;
Kyoo Hyung LEE
;
Jung Shin LEE
;
Woo Kun KIM
;
In Sik LEE
;
Ahm KIM
;
Jong Jin SEO
;
Hyun Sook CHI
Author Information
1. Department of Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Cord blood bank;
HLA;
Hematopoietic stem cell
- MeSH:
Bone Marrow;
Bone Marrow Transplantation;
Erythroid Precursor Cells;
Fetal Blood*;
Graft vs Host Disease;
Granulocyte-Macrophage Progenitor Cells;
Hematopoietic Stem Cells*;
Heparin;
Humans*;
Incidence;
Myeloid Progenitor Cells;
Placenta;
Syringes
- From:Korean Journal of Hematology
1998;33(2):171-176
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Because there are lower incidence of graft versus host disease in HLA mismatched cord blood transplantation compared to bone marrow transplantation, development of smaller scale cord blood bank could be possible. So we analysed the content of hematopoietic stem cell in cord blood and the distribution of HLA as a basic study for cord blood bank. METHODS: Seventy eight cord bloods were collected in heparinized bottle immediately after caesarian section. After expulsion of placenta, additional cord blood and placental blood were collected with heparinized syringe. Fifteen mL was sent to the laboratory for analysis and the rest was cryopreserved. RESULTS: The mean collected cord blood volume was 96.8mL (range, 55~163mL). And mean 81.8mL (range, 40~148mL) was cryopreserved. It contained mean 7.4x108 (range, 2.8x108~12.2x108) nucleated cells. In 2x105 mononuclear cells, 85 +/- 48 BFU-E, 19 +/- 17 CFU-E, 107 +/- 73 CFU-GM and 124 +/- 113 CFU-GEMM were present. With dextran/albumin thawing media, the viability of cryopreserved cord blood mononuclear cell was better than usual washing method with IMDM (82.3% vs. 74.6% P=0.004). Each cord blood could findHLA full matched, 5 loci matched and 4 loci matched cord blood in the remainders with the probability of 0, 11.9% and 58.4%. CONCLUSION: Development of more smaller scale cord blood bank could be possible compared to bone marrow bank.
