Variable Number of Tandem Repeats (VNTR) Disparity between Donor and Recipient has a Potential to Predict the Outcomes of HLA-identical Allogeneic Stem Cell Transplantation.
10.5045/kjh.2005.40.4.231
- Author:
Dong Hoon KWACK
1
;
Dong Hwan KIM
;
Shi Nae KIM
;
Byung Min AHN
;
Joon Ho MOON
;
Yee Soo CHAE
;
Jin Ho BAEK
;
Jong Gwang KIM
;
Sang Kyun SOHN
;
Nan Young LEE
;
Jang Soo SUH
;
Kyu Bo LEE
Author Information
1. Department of Hematology/Oncology, Kyungpook National University Hospital, Daegu, Korea. sksohn@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Variable number of tandem repeats;
Allogeneic stem cell transplantation;
Graft-versus-host disease
- MeSH:
Chimerism;
Chromosomes, Human, Pair 1;
Graft vs Host Disease;
Humans;
Incidence;
Minisatellite Repeats*;
Minor Histocompatibility Antigens;
Mortality;
Multivariate Analysis;
Polymerase Chain Reaction;
Siblings;
Stem Cell Transplantation*;
Stem Cells*;
Tissue Donors*;
Unrelated Donors
- From:Korean Journal of Hematology
2005;40(4):231-241
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Detection of variable number of tandem repeats (VNTR) between recipient and donor has been adopted to monitor the degree of chimerism after allogeneic stem cell transplantation (SCT). In allogeneic SCT, besides MHC-disparity, the disparity of various polymorphous proteins encoded by several genes may play a critical role in the pathogenesis of graft-versus-host disease (GVHD). However, the biologic effect of VNTR disparity has been scarcely studied. METHODS: We analyzed 84 patients receiving SCT from HLA-identical sibling (n=68) or unrelated donors (n=16). Enrolled diseases included AML 48, ALL 8, CML 15, NHL 10, and high-risk MDS 3. The PCR was performed to amplify 3 VNTR regions (D1S80, D1S111, and D17S5). RESULTS: We observed strong correlation between the D1S80 disparity and transplant outcomes in terms of OS (P=0.0179) or non-relapse mortality (NRM) (P=0.0305), but not for D1S111 or D17S5 disparity. The D1S80-fully matched pair showed a better OS (72% vs 38%) and lower NRM (17% vs 50%) compared to partially matched or mismatched pairs. In multivariate analyses, D1S80-fully matched pair was found to be independent favorable prognostic factor for OS (P=0.03) or NRM (P=0.05). In addition, the D1S80 disparity was significantly associated with the myeloid engraftment speed (P=0.01) or the occurrence of gut chronic GVHD (P=0.05). CONCLUSION: Our data suggest that disparities in D1S80-located on chromosome1-seemed to be associated with increased incidence of gut chronic GVHD and NRMs, thus suggesting the existence of unknown genes of minor histocompatibility antigens targeting gut or cytokine/cytokine receptor on chromosome 1.
