Induction of Cytotoxic T Lymphocytes by Dendritic Cells Pulsed with Murine Leukemic Cell RNA.
- Author:
Chul Won JUNG
1
;
Jung Hye KWON
;
Jae Goo SEOL
;
Woo Hyun PARK
;
Jung Mi HYUN
;
Eun Shil KIM
;
Seung Taik KIM
;
Sang Jae LEE
;
Byoung Kook KIM
;
Young Yiul LEE
Author Information
1. Division of Hematology Oncology1, Department of Medicine, Samsung Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Leukemia;
Dendritic cell;
Total RNA transfection;
Liposome
- MeSH:
Animals;
Bone Marrow;
Cell Line;
Cell Proliferation;
Colony-Stimulating Factors;
Dendritic Cells*;
Granulocyte-Macrophage Colony-Stimulating Factor;
Leukemia;
Liposomes;
Lymphocytes;
Mice;
RNA*;
T-Lymphocytes;
T-Lymphocytes, Cytotoxic*
- From:Korean Journal of Hematology
2001;36(3):223-231
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: This study was done to assess the feasibility of dendritic cell generation from murine bone marrow and the efficacy of dendritic cells pulsed with total RNA to induce specific cytotoxic T lymphocyte response against leukemic cells. METHODS: Nucleated cells of inbred BALB/c mice were obtained and cultured with granulocyte/macrophage colony-stimulating factor (GM-CSF) and lipopolysaccharide (LPS) to induce dendritic cells. Total RNA of WEHI-3BD+, a myelomonocytic leukemia cell line from BALB/c, was transfected into the dendritic cells using liposome. RNA pulsed dendritic cells were irradiated and administered to the BALB/c mice intraperitoneally and splenic T lymphocytes were harvested. After restimulation with leukemic cells, T cell proliferation and specific cytotoxicity was assessed. RESULTS: Cells cultured with GM-CSF and lipopolysaccaride were found to have prominent dendritic processes. The percentage of cells showing high expression of both MHC class II and CD80, CD86, or CD11c was 69.6 %, 63.7%, and 41.8%, respectively. T cells stimulated by WEHI-3BD+ total RNA pulsed dendritic cells using DOTAP showed enhanced proliferation than those stimulated by total RNA or media only (P=0.05). When T cells were cocultured with WEHI-3BD+ as target cells, T cells stimulated by WEHI-3BD+ total RNA pulsed dendritic cells using DOTAP showed much increased cytotoxicity than controls. CONCLUSION: Dendritic cells pulsed with total leukemic RNA could stimulate T cells to induce specific cytotoxic effect.
