Comparison of Immune Responses Induced by Deferoxamine and Deferasirox.
10.5045/kjh.2008.43.3.150
- Author:
Yeong Suk JUNG
1
;
E Young BAE
;
Nack Gyun CHUNG
;
Bin CHO
;
Hack Ki KIM
;
Chang Ki MIN
;
Chi Wha HAN
;
Ho Shik KIM
;
Dae Chul JEONG
Author Information
1. Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. dcjeong@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Deferoxamine;
Deferasirox;
Immunosuppressive effect
- MeSH:
Benzoates;
Cell Proliferation;
Cyclosporine;
Cytokines;
Deferoxamine;
Interleukin-10;
Interleukin-2;
Iron;
Iron Chelating Agents;
Lipopolysaccharides;
Spleen;
Triazoles
- From:Korean Journal of Hematology
2008;43(3):150-158
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The iron chelating agents (ICA) have various biological effects besides iron chelation. We investigated the immunomodulatory effects of Deferasirox (DFS) compared to Deferoxamine (DFO). METHODS: Spleen cells (SP) were obtained from 5 week-old C57/BL6 (H-2(b)). The cytotoxicity of ICAs was examined using the CCK8 method. For the cell proliferation assay, SP were cultured with irradiated in addition to 10, 50, 100micrometer of DFS or DFO and 200ng/mL of cyclosporin A (CSA). Cytokines and nitrite levels were evaluated from supernatants by ELISA. RESULTS: The viability of ICA was reported to be over 100%. Both DFS and DFO inhibited cell proliferation in a manner comparable to CSA. Cell proliferation without iron was reduced at the concentration of 100micrometer of DFO. With iron treatment, the reduction of the stimulation index was dependent on DFO concentrations. DFS decreased the proliferation without reference to the concentrations. After stimulation of phytohemagglutinin, the nitrite concentrations increased with iron. With lipopolysaccharides, the nitrite levels were higher in DFO with iron than control, but similar in DFS regardless of iron treatment. The levels of interleukin-2 were not different. Interleukin-10 was more abundantly produced in 50micrometer of DFO compared to DFS. Transforming growth factor-beta was higher in DFS than DFO at the low concentration, but opposite at the high concentration. CONCLUSION: These data suggested that both iron chelating agents possessed immune suppressive effects comparable to CSA. The immunosuppressive effect of DFS may be distinct from DFO. More experiments are required to determine the exact mechanism of the immunosuppressive effect of DFS.
