Eugenol Induces a Reactive Oxygen Species-mediated Apoptosis in HL-60 Human Promyelocytic Leukemia Cells.
- Author:
Uk Hyun KIL
1
;
Kee Hyun LEE
;
Kyung Tae LEE
;
Jong Youl JIN
Author Information
- Publication Type:Original Article
- Keywords: Eugenol; HL-60 cells; Apoptosis; Reactive oxygen species; Cytochrome c
- MeSH: Apoptosis*; Cell Death; Cytochromes c; Cytosol; Syzygium; Eugenol*; HL-60 Cells; Humans*; Leukemia*; Medicine, Traditional; Membrane Potential, Mitochondrial; Oxygen*; Permeability; Propidium; Reactive Oxygen Species; Rhodamine 123
- From:Korean Journal of Hematology 2005;40(2):65-74
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: Eugenol is a major component of the essential oil isolated from Eugenia caryophyllata (Myrtaceae), and has been widely used as a traditional medicine. In this study, the effects of eugenol on the cytotoxicity, induction of apoptosis and putative pathways of its actions were investigated in human promyelocytic leukemia cells (HL-60). METHODS: After applying eugenol to cultured HL-60, the changes in the mitochondrial membrane potential of the cells were monitored after double staining with propidium iodide and rhodamine 123, with 2', 7'-dicholorofluorescin diacetate was used to measure of levels of reactive oxygen species (ROS) RESULTS: Eugenol was shown to be a potent inducer of apoptosis; transducing the apoptotic signal via ROS generation; thereby, inducing mitochondrial permeability transition (MPT) and cytochrome c release to the cytosol. The production of ROS, mitochondrial alteration and subsequent apoptotic cell death in eugenol-treated cells were blocked by the antioxidant, N-acetylcystein (NAC). CONCLUSION: Taken together, the present study has demonstrated that eugenol induces ROS-mediated mitochondrial permeability transition and resultant cytochrome c release.
