A Case of DNA Chimerism Analysis as a Marker of Donor Lymphocyte Infusion.
- Author:
Tae Yeob KIM
1
;
Soo Hyang PARK
;
Jang Soo SUH
;
Sang Kyun SOHN
Author Information
1. Department of Clinical Pathology, School of Medicine, Kyungpook National University, Taegu, Korea. suhjs@knu.ac.kr
- Publication Type:Case Report
- Keywords:
DNA chimerism;
Mixed chimerism;
Variable number of tandem repeat (VNTR);
Allogeneic peripheral blood stem cell transplantation;
Donor lymphocyte infusion (DLI)
- MeSH:
Adult;
Chimerism*;
DNA*;
Genetic Markers;
Humans;
Leukemia;
Leukemia, Myeloid, Acute;
Lymphocytes*;
Male;
Minisatellite Repeats;
Recurrence;
Stem Cells;
Tissue Donors*
- From:Korean Journal of Hematology
2001;36(4):342-345
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Donor lymphocyte infusion (DLI) has some benefit effects as graft-versus-leukemia effect, reducing the relapse of leukemia and inducing of a complete remission. But it has also a graft-versus-host-disease (GVHD) effect. So it is required a proper marker test when DLI is performing. The DNA chimerism analysis can be a marker test in DLI. Variable number of tandem repeats (VNTR) are highly polymorphic DNA markers in the human genomic DNA and used as primers of DNA chimerism analysis. A 43-year-old male who had been diagnosed acute myelogenous leukemia was transplanted with allogeneic peripheral blood stem cells. The initial chimerism analysis showed complete chimerism but it changed to mixed chimerism after 7 months of transplantation. We predicted the relapse of leukemia and performed DLI. The patient could obtain the complete chimerism after DLI. We report a case of chimerism analysis which was useful to predict the relapse of leukemia and perform the DLI.
