Cytogenetical, Morphological, and Immunophenotypical Characteristics of Myeloid Malignancies with t (8;21).
- Author:
Kyeong Hee KIM
1
;
Tae Gyeom KIM
;
Jin Yeong HAN
;
Jung Man KIM
;
Jae Seok KIM
;
Hyo Jin KIM
;
Young Ho LEE
;
Eun Yup LEE
Author Information
1. Department of Clinical Pathology, Dong-A University College of Medicine, Pusan, Korea.
- Publication Type:Original Article
- Keywords:
Myeloid malignancies;
t (8;
21);
AML1-ETO;
CD19
- MeSH:
Anemia, Refractory;
Bone Marrow;
Cytogenetics;
Eosinophilia;
Humans;
Leukemia, Myeloid, Acute;
Myelodysplastic Syndromes;
Polymerase Chain Reaction;
Retrospective Studies;
Reverse Transcription;
Sarcoma, Myeloid;
Sex Chromosomes
- From:Korean Journal of Hematology
1999;34(1):18-26
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Myeloid malignancies carrying t (8;21) (q22;q22) exhibit several characteristic features. This translocation is most often associated with acute myeloid leukemia with maturation (AML-M2), but rarely with myelodysplastic syndrome. We studied the correlation of cytogenetics, morphology, and immunophenotype in 21 myeloid malignancies carrying t (8;21). METHODS: We analyzed 21 t (8;21) positive myeloid malignancies for morphology, immunophenotype and cytogenetics, retrospectively. We also performed reverse transcription polymerase chain reaction (RT-PCR) for AML1-ETO fusion transcripts using marrow slides stored at room temperature. RESULTS: 17 patients were diagnosed as French-American-British (FAB) type M2 and 4 cases as refractory anemia with excess blasts in transformation (RAEB-t). Three patients had marrow eosinophilia and 14 cases (67%) had Auer rods in leukemic blasts. Three patients (14%) developed granulocytic sarcomas. Four patients had variant t (8;21) translocation and 16 (76%) had secondary clonal abnormalities, most commonly loss of a sex chromosome (52%). 15 patients (83%) aberrantly expressed CD19. AML1-ETO fusion transcript of same size was observed in classic and variant t (8;21) translocation (11/14, 79%). CONCLUSION: Our study showed that myeloid malignancies carrying t (8;21) have distinctive morphological, immunophenotypical, and molecular features. These results can be aid to understand the pathogenesis and stratify treatment of those malignancies in the future.
