Detection of Maternal Cells in Human Umbilical Cord Blood Using PCR and Fluorescence In Situ Hybridization.
- Author:
Hyun Jung LEE
1
;
Bin CHO
;
Hack Ki KIM
Author Information
1. Department of Pediatrics, Catholic University Medical College, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Cord blood;
PCR;
FISH;
Contaminated with maternal cells
- MeSH:
Alleles;
Female;
Fetal Blood*;
Fluorescence*;
Graft vs Host Disease;
Hematopoietic Stem Cells;
Humans*;
In Situ Hybridization*;
Minisatellite Repeats;
Parturition;
Polymerase Chain Reaction*;
Umbilical Cord*
- From:Korean Journal of Hematology
1999;34(1):90-98
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Cord blood is a practical source of hematopoietic stem cells for transplantation. However, there are concerns that cord blood might be contaminated with maternal cells that could lead to graft-versus-host disease. MATERIAL AND METHODS: In the present study, we used the polymerase chain reaction (PCR) amplification of two minisatellite sequences (YNZ 22 and 33.6) and fluorescence in situ hybridization (FISH), to ascertain the presence of maternal cells in the cord blood. RESULTS: The results were as follows; 1) Maternal-specific allele was present in 1 of the 16 cord bloods (6.25%) by PCR. 2) We determined the sensitivity of this methods for detecting a maternal specific allele in the cord blood sample, which demonstrated as 0.2% (YNZ 22 and 33.6). 3) Maternal cells were found in 11 of the 20 cord bloods (55%), and 35 XX maternal cells were observed in 19,138 nuclei in the cord blood by FISH. 4) The range of maternal cells in the 11 positives was 0.1~8.6% and median value of maternal cells was 0.2%. CONCLUSION: These results suggest that maternal cells are very rarely present in the cord blood collected at birth. However, although small, this amount of cells may result in GVHD in a susceptible recipient. These methods we used allow the detection of maternal cells within cord blood from 104 nucleated cells by FISH but it was difficult to detect maternal cells within female cord blood by PCR, then it needs the use of a more reliable tool in a cord blood banking perspective.
