Effects of granulocyte-colony stimulating factor and the expression of its receptor on various malignant cells.
10.5045/kjh.2012.47.3.219
- Author:
Hee Won MOON
1
;
Tae Young KIM
;
Bo Ra OH
;
Sang Mee HWANG
;
Jiseok KWON
;
Ja Lok KU
;
Dong Soon LEE
Author Information
1. Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
G-CSF;
Differentiation;
Proliferation;
Solid tumor;
AML
- MeSH:
Breast;
Carcinoma, Squamous Cell;
Cell Line;
Cell Line, Tumor;
Flow Cytometry;
Granulocyte Colony-Stimulating Factor;
Larynx;
Melanoma;
Neutrophils;
Receptors, Granulocyte Colony-Stimulating Factor;
Recombinant Proteins;
RNA, Messenger
- From:Korean Journal of Hematology
2012;47(3):219-224
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Granulocyte-colony stimulating factor (G-CSF) is extensively used to improve neutrophil count during anti-cancer chemotherapy. We investigated the effects of G-CSF on several leukemic cell lines and screened for the expression of the G-CSF receptor (G-CSFR) in various malignant cells. METHODS: We examined the effects of the most commonly used commercial forms of G-CSF (glycosylated lenograstim and nonglycosylated filgrastim) on various leukemic cell lines by flow cytometry. Moreover, we screened for the expression of G-CSFR mRNA in 38 solid tumor cell lines by using real-time PCR. RESULTS: G-CSF stimulated proliferation (40-80% increase in proliferation in treated cells as compared to that in control cells) in 3 leukemic cell lines and induced differentiation of AML1/ETO+ leukemic cells. Among the 38 solid tumor cell lines, 5 cell lines (hepatoblastoma, 2 breast carcinoma, squamous cell carcinoma of the larynx, and melanoma cell lines) showed G-CSFR mRNA expression. CONCLUSION: The results of the present study show that therapeutic G-CSF might stimulate the proliferation and differentiation of malignant cells with G-CSFR expression, suggesting that prescreening for G-CSFR expression in primary tumor cells may be necessary before using G-CSF for treatment.