Evaluation of Antidepressant Drug Effect in a Depressive Animal Model by Proton MR Spectroscopy.
- Author:
Sang Young KIM
1
;
Chi Bong CHOI
;
Sung Ho LEE
;
Dong Cheol WOO
;
Seong Ik YOON
;
Kwan Soo HONG
;
Hyun Sung LEE
;
Chae Joon CHEONG
;
Bo Keun JEE
;
Sung Tak HONG
;
Hwi Yool KIM
;
Bo Young CHOE
Author Information
1. Department of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul, Korea. bychoe@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Forced swimming test (FST);
Desipramine-HCl;
Magnetic resonance spectroscopy (MRS);
Hippocampus
- MeSH:
Animals;
Choline;
Hippocampus;
Humans;
Magnetic Resonance Spectroscopy;
Male;
Models, Animal;
Protons;
Rats;
Rats, Sprague-Dawley;
Spectrum Analysis;
Swimming
- From:Korean Journal of Medical Physics
2008;19(2):95-101
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In this study, we observed the alteration of choline signal intensity in hippocampus region of the depressive rat model induced by forced swimming test (FST). The purpose of this study was to evaluate the antidepressant efficacy in the depressive animal model using MR spectroscopy. Fourteen experimentally naive male Sprague-Dawley rats weighting 160~180 g were used as subjects. Drug injection group was exposed to the FST except for control group. The drugs were administered subcutaneously (SC) in a volume equivalent to 2 ml/kg. And three injections were administered 23, 5, and 1 h before beginning the given test. 1H MR spectra were obtained with use of a point resolved spectroscopy (PRESS) localization sequence performed according to the following parameters: repetition time, 2500 ms; echo time, 144 ms; 512 average; 2048 complex data points; voxel dimensions, 1.5x2.5x2.5 mm3; acquisition time, 25 min. There were no differences in NAA/Cr and Cho/Cr ratio between the right and the left hippocampus both normal control rats and antidepressant-injected rats. Also, no differences were observed in NAA/Cr and Cho/Cr ratio between the normal control rats and the antidepressant-injected rats both the right and the left hippocampus. In this study, we found the recovery of choline signals in the depressive animal model similar to normal control groups as injecting desipramine-HCl which was antidepressant causing anti-immobility effects. Thus, we demonstrated that MR spectroscopy was able to aid in evaluating the antidepressant effect of desipramine-HCl.
