Studies on lactic dehydrogenase activity in parasitic helminths.
10.3347/kjp.1967.5.1.5
- Author:
Soon Hyung LEE
- Publication Type:Original Article
- MeSH:
parasitology-nematode-trematoda-helminth;
lacticdehydrogenase;
nicotinamide dinucloetide;
sodium pyruvate;
Ascaris lumbricoides;
Ascaridia galli;
Dirofilaria immitis;
Fasciola hepatica;
Eurytrema pancreaticum;
Paramphistomum sp.;
Clonorchis sinensis;
Paragonimus westermani;
Taenia saginata;
Taenia solium;
Taenia pisiformis;
Dipylidium caninum;
Diphyllobothrium mansoni;
sparganum, Cysticercus cellulosae;
Cysticercus fasciolaris;
biochemistry- enzyme
- From:The Korean Journal of Parasitology
1967;5(1):5-16
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
A series of experiments was performed to determine the lactic dehydrogenase activity of various parasitic helminths. The enzyme activity was determined by the modified method of Wroblewshi and LaDue (1955) using tissue homogenate of 16 kinds of worm parasites. The worms were mostly collected alive from local abattoir and removed from the organ or tissues of the naturally infected animal host and some materials were also obtained from the human host. They were thoroughly washed and homogenized in chilled glass tissue grinder, and then centrifuged. The supernatants were designated as enzyme preparations, and their enzyme activity was measured by spectrophotometry at the wave length of 340 millimicron. In order to know the effects of temperature and substrate concentration on the enzyme activity, the extinction of reduced Coenzyme I(NADH) was measured at the various conditions of incubation temperature and substrate concentration. The results of this experiments were as follows: The lactic dehydrogenase activity occurred over all kinds of parasites used in this study. Most worms of nematodes and trematodes displayed their maximum activity in the range of pH 2.7-3.5, and cestodes revealed their maximum activity in the ranges of both pH 2.7-3.5 and pH 7.4. In nematodes and trematodes, the lactic dehydrogenase activity increased slowly as incubation temperature increases except in the case of Eurytrema pancreaticum, while the activity in cestodes decreased inversely. The lactic dehydrogenase activity increased in proportion to the increase of substrate concentration in most of worm parasites.
