Cell Surface Antigen Display for Neuronal Differentiation-Specific Tracking.
10.4062/biomolther.2018.110
- Author:
Sang Chul KIM
1
;
Eun Hye LEE
;
Ji Hea YU
;
Sang Mi KIM
;
Bae Geun NAM
;
Hee Yong CHUNG
;
Yeon Soo KIM
;
Sung Rae CHO
;
Chang Hwan PARK
Author Information
1. Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Republic of Korea. chshpark@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Neuron specific;
Surface antigen;
Differentiation;
In vivo monitoring
- MeSH:
Antigens, Surface*;
Brain Diseases;
Neural Stem Cells;
Neurons*;
Stem Cells
- From:Biomolecules & Therapeutics
2019;27(1):78-84
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5′ UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.
