Effect of Korean Herbal Formula (Modified Ojayeonjonghwan) on Androgen Receptor Expression in an Aging Rat Model of Late Onset Hypogonadism.
- Author:
Sae Woong CHOI
1
;
Seung Hwan JEON
;
Eun Bi KWON
;
Guan Qun ZHU
;
Kyu Won LEE
;
Jin Bong CHOI
;
Hyun Cheol JEONG
;
Kang Sup KIM
;
Sang Rak BAE
;
Woong Jin BAE
;
Su Jin KIM
;
Hyuk Jin CHO
;
U Syn HA
;
Sung Hoo HONG
;
Sung Yeoun HWANG
;
Sae Woong KIM
Author Information
- Publication Type:Original Article
- Keywords: Hypogonadism; Phytotherapy; Receptors, androgen; Testosterone
- MeSH: Aging*; Animals; Blotting, Western; Epididymis; Hypogonadism*; Male; Models, Animal*; Oxidative Stress; Phytotherapy; Prescriptions; Rats*; Rats, Sprague-Dawley; Receptors, Androgen*; Superoxide Dismutase; Testis; Testosterone; Water; Weights and Measures
- From:The World Journal of Men's Health 2019;37(1):105-112
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: Testosterone replacement therapy is an effective treatment for late-onset hypogonadism (LOH) despite a few contraindications and side-effects. The aim of this study was to determine whether modified Ojayeonjonghwan (KH-204, Korean herbal formula) improved LOH. KH-204 is a strong antioxidant herbal formula. We evaluated the effect of Korean herbal prescription on androgen receptor (AR) expression in an aged rat model of LOH. MATERIALS AND METHODS: Eighteen-month-old rats were used as aged LOH rat models. Eighteen Sprague-Dawley rats were randomly divided into three equal groups of six animals each and treated with one of the following: 1) normal control group (oral administration with distilled water, n=6), 2) KH-204 200 group (oral administration with 200 mg/kg of KH-204, n=6), and 3) KH-204 400 group (oral administration with 400 mg/kg of KH-204, n=6). After four weeks of treatment (once daily, distilled water or KH-204), serum testosterone levels, changes in testicular and epididymal weight, Western blotting analysis of AR expression and measurement of oxidative stress were examined. RESULTS: Treatment with the herbal formulation KH-204 200 mg/kg and 400 mg/kg (1) increased the weights of testis and epididymis; (2) increased the level of serum testosterone; (3) increased the level of superoxide dismutase and reduced the level of 8-hydroxy-20-deoxyguanosine; and (4) upregulated AR expression in testicular tissue. CONCLUSIONS: KH-204 might be an effective alternative for LOH. It improves antioxidant mechanisms and increases testicular AR expression without side-effects.
