Feasibility and Stability of Liver Biopsy before Treatment for Preclinical Nonalcoholic Fatty Liver Studies.
- Author:
Yeon Ji CHAE
1
;
Dae Won JUN
;
Waqar Khalid SAEED
;
Hyeon Tae KANG
;
Ju Hee OH
;
Seung Min LEE
;
Kiseok JANG
Author Information
- Publication Type:Original Article
- Keywords: Animal Model; Nonalcoholic Fatty Liver Disease; Biopsy
- MeSH: Animals; Biopsy*; Body Weight; Diet; Diet, High-Fat; Diet, Western; Disease Progression; Eating; Inflammation; Liver*; Methionine; Mice; Models, Animal; Mortality; Non-alcoholic Fatty Liver Disease*; Population Characteristics
- From:Journal of Korean Medical Science 2019;34(2):e14-
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: The heterogeneity of histological findings in preclinical diet-induced nonalcoholic fatty liver disease (NAFLD) animal models is highly challenging. Here, we aimed to evaluate the feasibility and stability of repeated liver biopsy in NAFLD animal models. METHODS: Heterogeneity of diet-induced NAFLD was evaluated at different time points in 52 high-fat diet (HFD), 35 methionine choline-deficiency diet (MCD), and 166 western diet (WD) induced NAFLD mice. Serial liver biopsies (left lateral, right medial, and left medial lobes) were performed monthly for up to 3 months. Mortality rates and changes in food intake, body weight, and liver enzymes were assessed. RESULTS: At 12 weeks, of the HFD animals, 14% and 30% did not develop steatosis and lobular inflammation, respectively; of the MCD animals, 7% did not develop lobular inflammation; and of the WD animals, 14% and 51% did not develop steatosis and lobular inflammation, respectively. The mortality rate of repeated liver biopsy was 1.62% (2/123 mice died). Repeated liver biopsy can be used to trace disease progression. Although body weight, food intake, and liver enzymes slightly changed after biopsy, all recovered within a week. Repeated liver biopsy did not affect the degrees of inflammation and steatosis of the other liver lobes. CONCLUSION: The diet-induced NAFLD models were quite heterogeneous. Our results suggest that the repeated liver biopsy before treatment was applicable and stable in this NAFLD animal study.
