A Comparative Study of Apolipoprotein E Genotyping: INNO-LiPA ApoE Kit, Allelic Discrimination with LightCycler, and BioCore ApoE Kit.
- Author:
In Suk KIM
1
;
Hee Jin KIM
;
Chang Seok KI
;
Jong Won KIM
Author Information
1. Department of Laboratory Medicine, University of Sungkyunkwan School of Medicine and Samsung Medical Center, Seoul, Korea. jwonk@smc.samsung.co.kr
- Publication Type:Comparative Study ; Original Article
- Keywords:
Apolipoprotein E;
Alzheimer's disease;
Coronary artery disease;
INNO-LiPA;
Light-Cycler;
Amplification Refractory Mutation System
- MeSH:
Alzheimer Disease;
Apolipoproteins E*;
Apolipoproteins*;
Arm;
Cardiovascular Diseases;
Coronary Artery Disease;
Dementia;
Discrimination (Psychology)*;
Dyslipidemias;
Fluorescence Resonance Energy Transfer;
Genotype;
Real-Time Polymerase Chain Reaction;
Seoul
- From:The Korean Journal of Laboratory Medicine
2004;24(4):255-260
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Apolipoprotein E (apoE) polymorphism is associated with the risk and the time of onset of Alzheimer's disease and the risk of developing cardiovascular disease. Therefore, much inter-est in apoE genotyping has been focused both for epidemiological research and for the purpose of diagnosing dyslipoproteinemia or dementia. The aim of our study was to compare and evaluate three different methods for apoE genotyping. METHODS: We chose 10 samples each of six different apoE genotypes determined by INNO-LiPA ApoE Kit (INNOGENETICS, Gent, Belgium) used as the first comparison method. The other two were a fluorescence resonance energy transfer assay that used real-time PCR on the LightCycler instrument (Roche Diagnostics, Mannheim, Germany) and BioCore ApoE Kit (BioCore, Seoul, Korea) using the multiplex Amplification Refractory Mutation System (ARMS). RESULTS: All six genotypes for apoE were clearly discernible with INNO-LiPA ApoE Kit, allelic dis-crimination with LightCycler, and BioCore ApoE Kit. We obtained a 100% concordance rate among the three methods. It took approximately 6.5 hours, 70 minutes, and 3 hours, respectively, but hands-on time took 3 hours, 45 minutes, and 1 hour. CONCLUSIONS: The INNO-LiPA allelic specific oligonucleotide probe method is accurate and reliable, but labor intensive and relatively time consuming. The LightCycler allelic discrimination method seems to be rapid, simple and accurate, suggesting that it may be used successfully for diagnostic purposes. The BioCore multiplex ARMS analysis is reliable, simple to perform, and less time consuming; this method also may be appropriate for determining the apoE genotypes in clinical laboratories.
