Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes.
10.5625/lar.2018.34.4.147
- Author:
Hyeonhui KIM
1
;
Minki KIM
;
Sun Kyoung IM
;
Sungsoon FANG
Author Information
1. Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea. TJSRUD88@yuhs.ac, sfang@yuhs.ac
- Publication Type:Review
- Keywords:
Cre-loxP system;
tissue specific promoter;
Cre portal sites
- MeSH:
Animals;
Humans;
Mice*;
Recombinases
- From:Laboratory Animal Research
2018;34(4):147-159
- CountryRepublic of Korea
- Language:English
-
Abstract:
Genetically engineered mouse models are commonly preferred for studying the human disease due to genetic and pathophysiological similarities between mice and humans. In particular, Cre-loxP system is widely used as an integral experimental tool for generating the conditional. This system has enabled researchers to investigate genes of interest in a tissue/cell (spatial control) and/or time (temporal control) specific manner. A various tissue-specific Cre-driver mouse lines have been generated to date, and new Cre lines are still being developed. This review provides a brief overview of Cre-loxP system and a few commonly used promoters for expression of tissue-specific Cre recombinase. Also, we finally introduce some available links to the Web sites that provides detailed information about Cre mouse lines including their characterization.
