Development and Evaluation of Hyaluronic Acid-Based Hybrid Bio-Ink for Tissue Regeneration.
10.1007/s13770-018-0144-8
- Author:
Jaeyeon LEE
1
;
Se Hwan LEE
;
Byung Soo KIM
;
Young Sam CHO
;
Yongdoo PARK
Author Information
1. Department of Biomedical Engineering, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 02841, Republic of Korea. ydpark@kumc.or.kr
- Publication Type:Original Article
- Keywords:
Bio-ink;
Bioprinting;
Hyaluronic acid;
Hydrogel;
Tissue engineering
- MeSH:
Bioprinting;
Cues;
Fibroblasts;
Hyaluronic Acid;
Hydrogel;
Hydrogels;
Mesenchymal Stromal Cells;
Osteogenesis;
Osteopontin;
Peptides;
Regeneration*;
Regenerative Medicine;
Stem Cells;
Tissue Engineering
- From:
Tissue Engineering and Regenerative Medicine
2018;15(6):761-769
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Bioprinting has recently appeared as a powerful tool for building complex tissue and organ structures. However, the application of bioprinting to regenerative medicine has limitations, due to the restricted choices of bio-ink for cytocompatible cell encapsulation and the integrity of the fabricated structures. METHODS: In this study, we developed hybrid bio-inks based on acrylated hyaluronic acid (HA) for immobilizing bioactive peptides and tyramine-conjugated hyaluronic acids for fast gelation. RESULTS: Conventional acrylated HA-based hydrogels have a gelation time of more than 30 min, whereas hybrid bioink has been rapidly gelated within 200 s. Fibroblast cells cultured in this hybrid bio-ink up to 7 days showed < 90% viability. As a guidance cue for stem cell differentiation, we immobilized four different bio-active peptides: BMP-7-derived peptides (BMP-7D) and osteopontin for osteogenesis, and substance-P (SP) and Ac-SDKP (SDKP) for angiogenesis. Mesenchymal stem cells cultured in these hybrid bio-inks showed the highest angiogenic and osteogenic activity cultured in bio-ink immobilized with a SP or BMP-7D peptide. This bio-ink was loaded in a three-dimensional (3D) bioprinting device showing reproducible printing features. CONCLUSION: We have developed bio-inks that combine biochemical and mechanical cues. Biochemical cues were able to regulate differentiation of cells, and mechanical cues enabled printing structuring. This multi-functional bio-ink can be used for complex tissue engineering and regenerative medicine.
