Heat-Killed Saccharomyces cerevisiae, A Dectin-1 Agonist, Selectively Induces IgG4 Production by Human B Cells.
- Author:
Ha Yan PARK
1
;
Hee Kyung YOON
;
Jong Yeup KIM
;
Seok Rae PARK
Author Information
- Publication Type:Brief Communication
- Keywords: Heat-killed Saccharomyces cerevisiae; Human B cells; Dectin-1; Germline γ4 transcripts; IgG4
- MeSH: Animals; B-Lymphocytes*; Cell Line; Cell Wall; Humans*; Immunoglobulin Class Switching; Immunoglobulin G*; In Vitro Techniques; Mice; Saccharomyces cerevisiae*; Saccharomyces*
- From:Immune Network 2018;18(6):e46-
- CountryRepublic of Korea
- Language:English
- Abstract: Dectin-1 is a major receptor that recognizes fungal cell wall β-glucan. We previously reported that heat-killed Saccharomyces cerevisiae (HKSC), a Dectin-1 agonist, selectively induces IgG1 class switching in mouse B cells. Dectin-1 is also expressed on human B cells; however, Dectin-1 function in human B cells remains unknown. This study aimed to investigate the direct effect of in vitro stimulation using HKSC on Ig class switching in human B cells. HKSC selectively induced the expression of germline γ4 transcripts (GLTγ4) by human B cell line 2E2, and HKSC significantly augmented GLTγ4 promoter activity. Moreover, HKSC selectively enhanced GLTγ4 expression and IgG4 production by anti-CD40-activated human tonsillar resting B cells. Thus, these results suggest that Dectin-1 maybe involved in selective IgG4 class switching by human B cells.
