Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women.
10.5653/cerm.2018.45.4.189
- Author:
Pallop PONGSUTHIRAK
1
;
Teraporn VUTYAVANICH
Author Information
1. Department of Obstetrics and Gynecology, Buddhachinaraj Hospital Medical School, Phitsanulok, Thailand.
- Publication Type:Original Article
- Keywords:
Competence;
Oocyte in vitro maturation;
Pregnant women
- MeSH:
Blastocyst;
Cesarean Section;
Embryonic Development;
Female;
Fertilization;
Humans*;
In Vitro Oocyte Maturation Techniques;
Mental Competency*;
Needles;
Oocytes*;
Pregnancy;
Pregnant Women;
Sperm Injections, Intracytoplasmic
- From:Clinical and Experimental Reproductive Medicine
2018;45(4):189-194
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. METHODS: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later. Mature oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 hours. RESULTS: The percentage of degenerated oocytes was significantly higher (12.1% vs. 6.3%; p < 0.001) in group 1 than in group 2. There was no significant difference in the maturation rate (66.8% vs. 68.1%; p=0.698), fertilization rate (66.7% vs. 67.6%; p=0.857), or the rate of formation of good-quality blastocysts (46.2% vs. 47.2%; p=0.898) in oocytes obtained from pregnant and non-pregnant women. CONCLUSION: The developmental competence of immature oocytes did not differ between pregnant and non-pregnant women.
