Altered expression of MALAT1 lncRNA in chronic lymphocytic leukemia patients, correlation with cytogenetic findings.
- Author:
Abdolrahim AHMADI
1
;
Saeid KAVIANI
;
Marjan YAGHMAIE
;
Hossein PASHAIEFAR
;
Mohammad AHMADVAND
;
Mahdi JALILI
;
Kamran ALIMOGHADDAM
;
Mohammad ESLAMIJOUYBARI
;
Ardeshir GHAVAMZADEH
Author Information
- Publication Type:Original Article
- Keywords: MALAT1; Chronic lymphocytic leukemia; qRT-PCR; FISH
- MeSH: Chromosome Aberrations; Cytogenetics*; Fluorescence; Gene Expression; Humans; In Situ Hybridization; Leukemia, Lymphocytic, Chronic, B-Cell*; Polymerase Chain Reaction; Prognosis; Reverse Transcription; RNA, Long Noncoding*
- From:Blood Research 2018;53(4):320-324
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Recent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with cancer progression and prognosis. Thus, we here determined MALAT1 gene expression in chronic lymphocytic leukemia (CLL), a genetically heterogeneous disease, and explored its possible relationships with cytogenetic abnormalities. METHODS: MALAT1 expression level was evaluated using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on blood mononuclear cells from 30 non-treated CLL patients and 30 matched healthy controls. Cytogenetic abnormalities were determined in patients by fluorescence in situ hybridization (FISH). RESULTS: MALAT1 expression level was up-regulated in the CLL group compared to healthy controls (P=0.008). Del13q14, followed by Del11q22, were the most prevalent cytogenetic abnormalities. We found no association between the FISH results and MALAT1 expression in patients. CONCLUSION: Altered expression of MALAT1 is associated with CLL development. Further investigations are required to assess the relationship between this long non-coding RNA and CLL patient survival and prognosis.
