Immunohistochemical Profile of Acute Cellular Rejection in Renal Allograft.
- Author:
Jongha PARK
1
;
Seung Woon BYUN
;
Eunsil YU
;
Su Kil PARK
;
Duck Jong HAN
;
Yong Mee CHO
Author Information
1. Department of Nephrology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Korea.
- Publication Type:Original Article
- Keywords:
Renal transplantation;
Graft rejection;
Cellular immunity;
Immunohistochemistry
- MeSH:
Allografts*;
Biopsy;
Caspase 3;
Cytoplasm;
Diagnosis;
Epithelial Cells;
Fas Ligand Protein;
Graft Rejection;
Granzymes;
HLA-DR Antigens;
Humans;
Immunity, Cellular;
Immunohistochemistry;
Interferons;
Kidney Transplantation
- From:Korean Journal of Pathology
2007;41(1):15-20
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: We wanted to find an adjunctive marker(s) in renal allograft biopsies for predicting acute cellular rejection (ACR), and so the expression patterns of immune-related molecules were compared between ACR, borderline ACR and non-ACR cases. METHODS: The expression patterns of Fas ligand (FasL), HLA-DR, granzyme B, caspase-3, CD56, interferon stimulated growth factor-3 (ISGF-3), and CD53 were assessed via immunohistochemical study in 75 allograft biopsies from patients with ACR (n=19), borderline ACR (n=22), and non-ACR (n=34). RESULTS: Compared to those of the non-ACR group, the ACR group revealed an elevated number of FasL positive interstitial inflammatory cells, HLA-DR positive tubular inflammatory cells, cytoplasmic caspase-3 positive tubular epithelial cells, granzyme B positive interstitial mononuclear inflammatory cells and CD53 positive interstitial inflammatory cells. The expression patterns of the borderline ACR group were similar to those of non-ACR group, except for the intensity of FasL in the tubular epithelial cells. CONCLUSIONS: Immunohistochemical investigations of the adjunctive markers FasL, HLA-DR, granzyme B, caspase-3 and CD56 can be useful for making the diagnosis of ACR.
