Mucosal Immunity Related to FOXP3⁺ Regulatory T Cells, Th17 Cells and Cytokines in Pediatric Inflammatory Bowel Disease.
10.3346/jkms.2018.33.e336
- Author:
Jinhee CHO
1
;
Sorina KIM
;
Da Hee YANG
;
Juyeon LEE
;
Kyeong Won PARK
;
Junyong GO
;
Chang Lim HYUN
;
Youngheun JEE
;
Ki Soo KANG
Author Information
1. Department of Veterinary Medicine, Jeju National University, Jeju, Korea. yhjee@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
Inflammatory Bowel Disease;
FOXP3⁺;
Th17;
Cytokine
- MeSH:
Antibodies;
CD40 Ligand;
Child;
Colitis, Ulcerative;
Colon;
Crohn Disease;
Cytokines*;
Humans;
Ileum;
Immunity, Mucosal*;
Inflammatory Bowel Diseases*;
Interferons;
Interleukin-10;
Interleukin-17;
Interleukin-23;
Interleukin-33;
Interleukin-4;
Interleukin-6;
Interleukins;
Mucous Membrane;
Necrosis;
T-Lymphocytes;
T-Lymphocytes, Regulatory*;
Th17 Cells*
- From:Journal of Korean Medical Science
2018;33(52):e336-
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: We aimed to investigate mucosal immunity related to forkhead box P3 (FOXP3+) regulatory T (Treg) cells, T helper 17 (Th17) cells and cytokines in pediatric inflammatory bowel disease (IBD). METHODS: Mucosal tissues from terminal ileum and colon and serum samples were collected from twelve children with IBD and seven control children. Immunohistochemical staining was done using anti-human FOXP3 and anti-RORγt antibodies. Serum levels of cytokines were analyzed using a multiplex assay covering interleukin (IL)-1β, IL-4, IL-6, IL-10, IL-17A/F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, interferon (IFN)-γ, soluble CD40L, and tumor necrosis factor-α. RESULTS: FOXP3+ Treg cells in the lamina propria (LP) of terminal ileum of patients with Crohn's disease were significantly (P < 0.05) higher than those in the healthy controls. RORγt+ T cells of terminal ileum tended to be higher in Crohn's disease than those in the control. In the multiplex assay, serum concentrations (pg/mL) of IL-4 (9.6 ± 1.5 vs. 12.7 ± 3.0), IL-21 (14.9 ± 1.5 vs. 26.4 ± 9.1), IL-33 (14.3 ± 0.9 vs. 19.1 ± 5.3), and IFN-γ (15.2 ± 5.9 vs. 50.2 ± 42.4) were significantly lower in Crohn's disease than those in the control group. However, serum concentration of IL-6 (119.1 ± 79.6 vs. 52.9 ± 39.1) was higher in Crohn's disease than that in the control. Serum concentrations of IL-17A (64.2 ± 17.2 vs. 28.3 ± 10.0) and IL-22 (37.5 ± 8.8 vs. 27.2 ± 3.7) were significantly higher in ulcerative colitis than those in Crohn's disease. CONCLUSION: Mucosal immunity analysis showed increased FOXP3+ T reg cells in the LP with Crohn's disease while Th17 cell polarizing and signature cytokines were decreased in the serum samples of Crohn's disease but increased in ulcerative colitis.
