Characterization of Brachyspira hyodysenteriae isolates from Korea.
- Author:
Tae Jung KIM
1
;
Suk Chan JUNG
;
Jae Il LEE
Author Information
1. College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea. jaeil@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Brachyspira hyodysenteriae;
multilocus enzyme electrophoresis;
Korea;
serotype;
swine dysentery
- MeSH:
Animals;
Electrophoresis;
Genes, Bacterial;
Korea/epidemiology;
Rabbits;
Serotyping;
Serpulina hyodysenteriae/classification/genetics/*isolation&purification;
Spirochaetales Infections/*microbiology;
Swine/microbiology;
Swine Diseases/*microbiology;
Variation (Genetics)
- From:Journal of Veterinary Science
2005;6(4):335-339
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was done to characterize diversity in 10 Brachyspira hyodysenteriae isolates in Korea. The isolates were compared with 14 well-characterized non-Korean strains of various Brachyspira species. All Korean isolates showed strong beta haemolysis and had blunt cell ends with 7~14 periplasmic flagella. They produced indole, and did not ferment fructose. They were alpha-glucosidase positive and alpha-galatosidase negative using the APIZYM kit. Using polyclonal antisera raised in rabbits against recognized serotypes, all isolates showed a strong reaction to B. hyodysenteriae antisera E, A and B. Using multilocus enzyme electrophoresis (MLEE) with 15 enzymes and 5 buffer systems, the Korean and non-Korean isolates were divided into 22 electrophoretic types (ETs) and 5 divisions (A, B, C, D and E). Division A corresponded to B. hyodysenteriae, B to B. innocens, C to B. intermedia, D to B. murdochii and E to B. pilosicoli. The 10 Korean isolates of B. hyodysenteriae were relatively diverse, being divided into 9 ETs within MLEE division A. They were all distinct from the non-Korean strains.
