Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice.
- Author:
Eun Jin MOON
1
;
Il Gyu KO
;
Sung Eun KIM
;
Jun Jang JIN
;
Lakkyong HWANG
;
Chang Ju KIM
;
Hyeonjun AN
;
Bong Jae LEE
;
Jae Woo YI
Author Information
- Publication Type:Original Article
- Keywords: Sleep deprivation; Dexmedetomidine; Depression; Serotonin; Dopamine
- MeSH: Animals; Blotting, Western; Depression; Dexmedetomidine*; Dopamine; Hindlimb Suspension; Immunohistochemistry; Mice*; Physical Exertion; Receptors, Dopamine; Serotonin; Sleep Deprivation; Tryptophan Hydroxylase; Tyrosine 3-Monooxygenase; Up-Regulation; Water
- From:International Neurourology Journal 2018;22(Suppl 3):S139-S146
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: Sleep deprivation induces depressive symptoms. Dexmedetomidine is a α2-adrenoreceptor agonist and this drug possesses sedative, anxiolytic, analgesic, and anesthetic-sparing effect. In this study, the action of dexmedetomidine on sleep deprivation-induced depressive behaviors was investigated using mice. METHODS: For the inducing of sleep deprivation, the mice were placed inside a water cage containing 15 platforms and filled with water up to 1 cm below the platform surface for 7 days. One day after sleep deprivation, dexmedetomidine at the respective dosage (0.5, 1, and 2 μg/kg) was intraperitoneally treated into the mice, one time per a day during 6 days. Then, forced swimming test and tail suspension test were conducted. Immunohistochemistry for tyrosine hydroxylase (TH), 5-hydroxytryptamine (5-HT; serotonin), tryptophan hydroxylase (TPH) and western blot for D1 dopamine receptor were also performed. RESULTS: Sleep deprivation increased the immobility latency in the forced swimming test and tail suspension test. The expressions of TPH, 5-HT, and D1 dopamine receptor were decreased, whereas, TH expression was increased by sleep deprivation. Dexmedetomidine decreased the immobility latency and increased the expressions of TPH, 5-HT, and D1 dopamine receptor, whereas, HT expression was decreased by dexmedetomidine treatment. CONCLUSIONS: In our results, dexmedetomidine alleviated sleep deprivation-induced depressive behaviors by increasing 5-HT synthesis and by decreasing dopamine production with up-regulation of D1 dopamine receptor.
