Herpes Zoster DNA Vaccines with IL-7 and IL-33 Molecular Adjuvants Elicit Protective T Cell Immunity.
- Author:
A Reum KIM
1
;
Junsik PARK
;
Jong Hoon KIM
;
Jeong Eun KWAK
;
Youngran CHO
;
Hyojin LEE
;
Moonsup JEONG
;
Su Hyung PARK
;
Eui Cheol SHIN
Author Information
- Publication Type:Original Article
- Keywords: Herpes zoster; T-cells; DNA vaccines; IL-7; IL-33
- MeSH: Aging; Animals; DNA*; Ganglia, Sensory; Glycoproteins; Herpes Zoster*; Herpesvirus 3, Human; Immunosuppression; Interleukin-33*; Interleukin-7*; Mice; Plasmids; T-Lymphocytes; Vaccination; Vaccines, DNA*; Vaccinia virus
- From:Immune Network 2018;18(5):e38-
- CountryRepublic of Korea
- Language:English
- Abstract: Herpes zoster (HZ), or shingles, is caused by the reactivation of latent varicella-zoster virus (VZV) from the sensory ganglia when VZV-specific T-cell immunity is decreased because of aging or immunosuppression. In the present study, we developed HZ DNA vaccine candidates encoding VZV proteins and cytokine adjuvants, such as IL-7 and IL-33. We immunized C57BL/6 mice with DNA plasmids encoding VZV glycoprotein E (gE), immediate early (IE) 63, or IE62 proteins and found that robust VZV protein-specific T-cell responses were elicited by HZ DNA vaccination. Co-administration of DNA plasmids encoding IL-7 or IL-33 in HZ DNA vaccination significantly enhanced the magnitude of VZV protein-specific T-cell responses. Protective immunity elicited by HZ DNA vaccination was proven by challenge experiments with a surrogate virus, vaccinia virus expressing gE (VV-gE). A single dose of HZ DNA vaccine strongly boosted gE-specific T-cell responses in mice with a history of previous infection by VV-gE. Thus, HZ DNA vaccines with IL-7 and IL-33 adjuvants strongly elicit protective immunity.
