Cellular Response of Anodized Titanium Surface by Poly(Lactide-co-Glycolide)/Bone Morphogenic Protein-2.
10.1007/s13770-018-0137-7
- Author:
Su Young LEE
1
;
Jai Young KOAK
;
Seong Kyun KIM
;
Seong Joo HEO
Author Information
1. Department of Prosthodontics, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Bone morphogenic protein;
Polymer;
Anodized titanium;
Cell proliferation;
Cell differentiation
- MeSH:
Alkaline Phosphatase;
Cell Differentiation;
Cell Proliferation;
Fluorescent Antibody Technique;
Humans;
Polymers;
RNA, Messenger;
Sarcoma;
Titanium*
- From:
Tissue Engineering and Regenerative Medicine
2018;15(5):591-599
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The purpose of this study is to examine physical characteristics of and initial biological properties to anodized titanium treated with poly(D,L-lactide-co-glycolide) (PLG) mixed with recombinant human bone morphogenic protein-2 (rhBMP-2). METHODS: Titanium specimens were prepared in groups of four as follows: group NC was anodized under 300 V as control; group PC was anodized then dropped and dried with solution 0.02 ml PLG; group D was anodized then dropped and dried with solution 0.02 ml PLG/rhBMP-2 (3.75 µg per disc); and group E was anodized then coated with 0.02 ml PLG/rhBMP-2 (3.75 µg per disc) by electrospray. Human osteoblastic-like sarcoma cells were cultured. Cell proliferation and alkaline phosphatase (ALP) activity test were carried out. Runx-2 gene was investigated by the reverse transcription-polymerase chain reaction. Immunofluorescence outcome of osteogenic proteins was observed. RESULTS: After 3 days, there were significantly higher proliferations compared rhBMP-2 loaded titanium discs with rhBMP-2 unloaded discs. The ALPase activity on rhBMP-2 loaded titanium discs was significantly higher than in rhBMP-2 unloaded discs. The expression level of Runx2 mRNA presented the highest on the PLG/rhBMP-2-coated surface. CONCLUSION: PLG polymers mixed with rhBMP-2 might improve proliferation, differentiation and osteogenic protein formation of cells on the anodized titanium.
