Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics.
10.1007/s13770-018-0133-y
- Author:
Na Hee YU
1
;
So Young CHUN
;
Yun Sok HA
;
Hyun Tae KIM
;
Dae Hwan KIM
;
Jeongshik KIM
;
Jae Wook CHUNG
;
Jun Nyung LEE
;
Phil Hyun SONG
;
Eun Sang YOO
;
Bum Soo KIM
;
Tae Gyun KWON
Author Information
1. Biomedical Research Institute, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu 41944, Korea.
- Publication Type:Original Article
- Keywords:
Cell transporting;
Medium;
Cell density;
Amniotic fluid stem cell
- MeSH:
Amniotic Fluid;
Cell Count;
Cell Survival*;
Cell- and Tissue-Based Therapy;
Female;
Glass;
Humans;
Stem Cells*;
Survival Rate;
Syringes
- From:
Tissue Engineering and Regenerative Medicine
2018;15(5):639-647
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells' (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 ℃), cell density (1 × 10⁷ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 ℃, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport.
