Circular RNA-ZFR Inhibited Cell Proliferation and Promoted Apoptosis in Gastric Cancer by Sponging miR-130a/miR-107 and Modulating PTEN.
- Author:
Tonglei LIU
1
;
Shuang LIU
;
Yu XU
;
Ruo SHU
;
Feng WANG
;
Cheng CHEN
;
Yujian ZENG
;
Huayou LUO
Author Information
- Publication Type:Original Article
- Keywords: Circ-ZFR; miR-130a; miR-107; PTEN; Stomach neoplasms
- MeSH: Animals; Apoptosis*; Blotting, Western; Cell Count; Cell Cycle; Cell Cycle Checkpoints; Cell Proliferation*; Computational Biology; Flow Cytometry; Gene Expression; Heterografts; Immunoprecipitation; Mice; Polymerase Chain Reaction; PTEN Phosphohydrolase; Reverse Transcription; RNA; Stomach Neoplasms*
- From:Cancer Research and Treatment 2018;50(4):1396-1417
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: This study aimed to probe into the associations among circular RNA ZFR (circ-ZFR), miR-130a/miR-107, and PTEN, and to investigate the regulatory mechanism of circ-ZFR–miR-130a/miR-107–PTEN axis in gastric cancer (GC). MATERIALS AND METHODS: GSE89143 microarray data used in the study were acquired from publicly available Gene Expression Omnibus database to identify differentially expressed circular RNAs inGC tissues. The expressions of circ-ZFR, miR-130a, miR-107, and PTEN were examined by real-time reverse transcription polymerase chain reaction, while PTEN protein expression was measured by western blot. The variation of GC cell proliferation and apoptosis was confirmed by cell counting kit-8 assay and flow cytometry analysis. The targeted relationships among circZFR, miR-130a/miR-107, and PTEN were predicted via bioinformatics analysis and demonstrated by dual-luciferase reporter assay and RNA immunoprecipitation assay. The impact of ZFR on gastric tumor was further verified in xenograft mice model experiment. RESULTS: Circ-ZFR and PTEN were low-expressed whereas miR-107 and miR-130a were highexpressed in GC tissues and cells. There existed targeted relationships and interactions between miR-130a/miR-107 and ZFR/PTEN. Circ-ZFR inhibited GC cell propagation, cell cycle and promoted apoptosis by sponging miR-107/miR-130a, while miR-107/miR-130a promoted GC cell propagation and impeded apoptosis through targeting PTEN. Circ-ZFR inhibited cell proliferation and facilitated apoptosis in GC by sponging miR-130a/miR-107 and modulating PTEN. Circ-ZFR curbed GC tumor growth and affected p53 protein expression in vivo. CONCLUSION: Circ-ZFR restrained GC cell proliferation, induced cell cycle arrest and promoted apoptosis by sponging miR-130a/miR-107 and regulating PTEN.
