Comparative Study of the Effects of Different Growth Hormone Doses on Growth and Spatial Performance of Hypophysectomized Rats.
10.3346/jkms.2009.24.4.729
- Author:
Min Jung KWAK
1
;
Hee Ju PARK
;
Mi Hyun NAM
;
O Suk KWON
;
So Young PARK
;
So Yeon LEE
;
Mi Jin KIM
;
Su Jin KIM
;
Kyung Hoon PAIK
;
Dong Kyu JIN
Author Information
1. Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. jindk@skku.edu
- Publication Type:Comparative Study
- Keywords:
Growth Hormone;
Hypophysectomized Rat;
Somatic Growth;
Cognitive Function;
Morris Water Maze Test
- MeSH:
Animals;
Body Weight;
Female;
Growth/*drug effects;
Growth Plate/drug effects/pathology;
Human Growth Hormone/administration & dosage/*pharmacology;
Humans;
*Hypophysectomy;
Rats;
Rats, Sprague-Dawley;
Spatial Behavior/*drug effects
- From:Journal of Korean Medical Science
2009;24(4):729-736
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was designed to examine the effects of recombinant human growth hormone replacement on somatic growth and cognitive function in hypophysectomized (HYPOX) female Sprague-Dawley rats. Rats (5 per group) were randomized by weight to 3 experimental groups: group 1, administered 200 microgram/kg of GH once daily for 9 days; group 2, administered 200 microgram/kg of GH twice daily; and group 3, administered saline daily. Somatic growth was evaluated by measurement of body weight daily and of the width of the proximal tibial growth plate of the HYPOX rats. Cognitive function was evaluated using the Morris water maze (MWM) test. The results indicated that GH replacement therapy in HYPOX rats promoted an increase in the body weight and the width of the tibial growth plate in a dose-dependent manner. On the third day of the MWM test, the escape latency in the GH-treated groups 1 and 2 was significantly shorter than that in the control rats (P<0.001 and P=0.032, respectively), suggesting that rhGH improved spatial memory acquisition in the MWM test. Therefore it is concluded that rhGH replacement therapy in HYPOX rats stimulates an increase in somatic growth in a dose-dependent manner and also has beneficial effects on cognitive functions.
