IgE Cross-Reactivity between Humulus japonicus and Humulus lupulus.
10.3349/ymj.2018.59.7.852
- Author:
Kyoung Yong JEONG
1
;
Jongsun LEE
;
Gianni MISTRELLO
;
Kyung Hee PARK
;
Jung Won PARK
Author Information
1. Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea. parkjw@yuhs.ac, jeongky@yuhs.ac
- Publication Type:Original Article
- Keywords:
Allergen;
cross-reactivity;
Japanese hop;
pollinosis;
pollen
- MeSH:
Allergens;
Asian Continental Ancestry Group;
Chromatography;
Electrophoresis, Gel, Two-Dimensional;
Enzyme-Linked Immunosorbent Assay;
Equipment and Supplies;
Far East;
Humans;
Humulus*;
Hypersensitivity;
Immunoblotting;
Immunoglobulin E*;
Indicators and Reagents;
Mass Spectrometry;
Plants;
Pollen;
Rhinitis, Allergic, Seasonal;
Tandem Mass Spectrometry
- From:Yonsei Medical Journal
2018;59(7):852-856
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Japanese hop (Humulus japonicus) is a major cause of weed pollinosis in East Asia. However, supplies of commercial allergen extract from this plant have not met clinical demand. The pollen of common hop (Humulus lupulus), a closely related species, may provide an alternative source if there is strong IgE cross-reactivity between these two species. We aimed to compare the IgE cross-reactivity and allergenicity of common hop and Japanese hop pollen. MATERIALS AND METHODS: Cross-reactivity was measured by inhibition ELISA. One- and two-dimensional (2D) gel analyses combined with IgE immunoblotting and mass spectrometry [liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)] were performed to detect IgE-reactive pollen components. RESULTS: Up to 16.7% of IgE reactivity to Japanese hop was inhibited by common hop. A 12-kDa protein component of Japanese hop pollen that showed the most potent IgE reaction was absent from common hop. Six IgE-reactive components from Japanese hop were detected by 2D gel electrophoresis and LC-ESI-MS/MS, but showed low Mascot scores, preventing positive identification. CONCLUSION: No significant IgE cross-reaction was observed for Japanese and common hop pollen allergens. Development of allergy diagnostic and immunotherapeutic reagents based on Japanese hop pollen are urgently needed.
