Angiopoietin-1 and -2 and vascular endothelial growth factor expression in ovarian grafts after cryopreservation using two methods.
10.5653/cerm.2018.45.3.143
- Author:
In Ae CHO
1
;
Yeon Jee LEE
;
Hee Jung LEE
;
In Young CHOI
;
Jeong Kyu SHIN
;
Soon Ae LEE
;
Jong Hak LEE
;
Won Jun CHOI
Author Information
1. Department of Obstetrics and Gynecology, Gyeongsang National University Hospital, Jinju, Korea. choiwj@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Angiopoietin-1;
Angiopoietin-2;
Autografts;
Cryopreservation;
Fertility preservation;
Mice;
Neovascularization;
Ovary;
Reverse transcriptase polymerase chain reaction;
Vascular endothelial growth factors
- MeSH:
Angiopoietin-1*;
Angiopoietin-2;
Animals;
Anoxia;
Autografts;
Cryopreservation*;
Dimethyl Sulfoxide;
Female;
Fertility;
Fertility Preservation;
Ficoll;
Freezing;
Humans;
Methods*;
Mice;
Mice, Inbred ICR;
Ovary;
Reverse Transcriptase Polymerase Chain Reaction;
RNA, Messenger;
Sucrose;
Survivors;
Tissue Transplantation;
Transplantation, Autologous;
Transplants*;
Vascular Endothelial Growth Factor A*;
Vascular Endothelial Growth Factors;
Vitrification
- From:Clinical and Experimental Reproductive Medicine
2018;45(3):143-148
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The favored method of preserving fertility in young female cancer survivors is cryopreservation and autotransplantation of ovarian tissue. Reducing hypoxia until angiogenesis takes place is essential for the survival of transplanted ovarian tissue. The aim of this study was to investigate the role of angiopoietin-1 (Angpt-1), angiopoietin-2 (Angpt-2), and vascular endothelial growth factor (VEGF) in ovarian tissue grafts that were cryopreserved using two methods. METHODS: Ovarian tissues harvested from ICR mice were divided into three groups: group I (control), no cryopreservation; group II, vitrification in EFS (ethylene-glycol, ficoll, and sucrose solution)-40; and group III, slow freezing in dimethyl sulfoxide. We extracted mRNA for VEGF, Angpt-1, and Angpt-2 from ovarian tissue 1 week following cryopreservation and again 2 weeks after autotransplantation. We used reverse transcriptase-polymerase chain reaction to quantify the levels of VEGF, Angpt-1, and Angpt-2 in the tissue. RESULTS: Angpt-1 and Angpt-2 expression decreased after cryopreservation in groups II and III. After autotransplantation, Angpt-1 and Angpt-2 expression in ovarian tissue showed different trends. Angpt-1 expression in groups II and III was lower than in group I, but Angpt-2 in groups II and III showed no significant difference from group I. The vitrified ovarian tissues had higher expression of VEGF and Angpt-2 than the slowfrozen ovarian tissues, but the difference was not statistically significant. CONCLUSION: Our results indicate that Angpt-2 may play an important role in ovarian tissue transplantation after cryopreservation although further studies are needed to understand its exact function.
