Comparison of ELISA and Urine Microscopy for Diagnosis of Schistosoma haematobium Infection.
10.3346/jkms.2018.33.e238
- Author:
Hyun Beom SONG
1
;
Jiyoung KIM
;
Yan JIN
;
Jin Soo LEE
;
Hoo Gn JEOUNG
;
Young Ha LEE
;
Abd Al Wahab SAEED
;
Sung Tae HONG
Author Information
1. Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul, Korea. hst@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Urogenital Schistosomiasis;
Schistosoma haematobium;
Diagnosis;
Urine Microscopy;
ELISA
- MeSH:
Antibodies;
Diagnosis*;
Enzyme-Linked Immunosorbent Assay*;
Immunoglobulins;
Mass Screening;
Methods;
Microscopy*;
Ovum;
Parasites;
Schistosoma haematobium*;
Schistosoma japonicum;
Schistosoma mansoni;
Schistosoma*;
Schistosomiasis haematobia;
Sensitivity and Specificity;
Sudan
- From:Journal of Korean Medical Science
2018;33(33):e238-
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Schistosoma haematobium which causes urogenital schistosomiasis (UGS) is highly prevalent in African countries. Urine microscopy (UM) is the first-line diagnostic method of UGS. Enzyme-linked immunosorbent assay (ELISA) is a common method for screening many parasite infections primarily or alternatively. The present study established an in-house diagnostic system by ELISA and evaluated its diagnostic efficacy in comparison with UM for screening UGS in White Nile State, Republic of Sudan, 2011–2013. METHODS: A total of 490 participants were screened by UM or ELISA, and 149 by both. The in-house ELISA system was established employing soluble egg antigen of S. haematobium and the cut-off absorbance was set at 0.270. RESULTS: Of the 149 subjects, 58 participants (38.9%) were positive by UM, 119 (79.9%) were positive by ELISA and 82 (55.0%) showed consistently positive or negative results by both methods. The diagnostic sensitivity of ELISA was 94.8% and specificity was 29.7% based on UM results. The ELISA positive serum samples also cross-reacted with egg antigens of Schistosoma mansoni and Schistosoma japonicum. CONCLUSION: We have established in-house ELISA for screening serum immunoglobulin (Ig) G antibodies by employing soluble egg antigen of S. haematobium for diagnosis of UGS with 94.8% sensitivity and 29.7% specificity. The ELISA system can supplement the conventional diagnosis by UM.
