Thapsigargin Increases IL-2 Production in T Cells at Nanomolar Concentrations.
- Author:
Ki Hyang KIM
1
;
Sang Hyun KIM
;
Ho Hyun JUNG
;
Jun Hyeok MOON
;
Seong Un JEONG
;
Kyeongae YU
;
Chong Kil LEE
Author Information
- Publication Type:Brief Communication
- Keywords: Thapsigargin; Cytoplasmic Ca²⁺; T cells; IL-2; Phorbol myristate acetate
- MeSH: Cell Proliferation; Concanavalin A; Cytokines; Cytoplasm; Dendritic Cells; Interferon-gamma; Interleukin-2*; Interleukin-4; Macrophages; T-Lymphocytes*; Tetradecanoylphorbol Acetate; Thapsigargin*
- From:Immune Network 2018;18(4):e26-
- CountryRepublic of Korea
- Language:English
- Abstract: Thapsigargin (TGN) is a potent and selective inhibitor of sarco-endoplasmic Ca²⁺-ATPase, leading to rapid elevation of cytoplasmic Ca2+ concentration. Previous reports have shown that TGN increases the production of various cytokines from macrophages and dendritic cells. Here, we examine the effects of TGN on murine T cells. Nanomolar concentrations of TGN are a significant inducer of IL-2 production with full activity at 50 nM. Micromolar concentrations of TGN, however, are inhibitory to IL-2 production and T cell proliferation. The IL-2 production-inducing activity of TGN is much more prominent when T cells are primed with concanavalin A or anti-CD3 mAb, and is due to the increase of cytoplasmic Ca²⁺ concentration. TGN at 50 nM does not affect interferon-gamma or IL-4 production from T cells. Thus, the present study shows that low nanomolar concentrations of TGN could be useful in potentiating IL-2 production from antigen-primed T cells.
