Interleukin-4 Contributes to Degeneration of Dopamine Neurons in the Lipopolysaccharide-treated Substantia Nigra in vivo.
- Author:
Eugene BOK
1
;
Eun Ju CHO
;
Eun Sook CHUNG
;
Won Ho SHIN
;
Byung Kwan JIN
Author Information
- Publication Type:Original Article
- Keywords: Interleukin-4; Parkinson disease; Substantia nigra; Dopaminergic neurons; Lipopolysaccharides
- MeSH: Animals; Antibodies, Neutralizing; Astrocytes; Blood-Brain Barrier; Dopamine*; Dopaminergic Neurons*; Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Interleukin-4*; Interleukins; Lipopolysaccharides; Microglia; Neurons; Neuroprotection; Parkinson Disease; Rats; Substantia Nigra*; Tyrosine 3-Monooxygenase
- From:Experimental Neurobiology 2018;27(4):309-319
- CountryRepublic of Korea
- Language:English
- Abstract: The present study investigated the effects of interleukin (IL)-4 on dopamine (DA) neurons in the substantia nigra (SN) in vivo of lipopolysaccharide (LPS)-treated rat. Tyrosine hydroxylase immunohistochemistry showed a significant loss of nigral DA neurons at 3 and 7 day post-LPS. In parallel, IL-4 immunoreactivity was upregulated as early as 1 day, reached a peak at 3 day and remained elevated at 7 day post-LPS. IL-4 immunoreactivity was detected exclusively in microglia. IL-4 neutralizing antibody (NA) significantly increased survival of DA neurons in LPS-treated SN in vivo by inhibiting microglial activation and production of proinflammatory mediator such as IL-1β as assessed by immunihistochemical, RT-PCR and ELISA analysis, respectively. Accompanying neuroprotection are IL-4NA effects on decreased disruption of blood-brain barrier and astrocytes. The present data suggest that endogenously expressed IL-4 from reactive microglia may be involved in the neuropathological processes of degeneration of DA neurons occurring in Parkinson's disease.
