Anti-inflammatory activities of Scolopendra subspinipes mutilans in RAW 264.7 cells.
10.4163/jnh.2018.51.4.323
- Author:
Jae Hyeon PARK
1
;
Sun Ryung LEE
Author Information
1. Department of Biology, Jeju National University, Jeju 63243, Korea. srlee@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
inflammation;
nitric oxide;
NF-κB;
cytokine;
Scolopendra subspinipes mutilans
- MeSH:
Blotting, Western;
Cytokines;
Cytosol;
Inflammation;
Interleukin-6;
Nitric Oxide;
Nitric Oxide Synthase;
RAW 264.7 Cells*;
RNA, Messenger;
Up-Regulation
- From:Journal of Nutrition and Health
2018;51(4):323-329
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The dried body of Scolopendra subspinipes mutilans has long been used as a traditional Korean medicinal food, but little is known about its mechanisms of action. In this study, we investigated the anti-inflammatory activities of Scolopendra subspinipes mutilans and possible mechanisms in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. METHODS: Cytotoxicity of Scolopendra subspinipes mutilans extract (SSME) was measured by MTT assay, anti-inflammatory activities were analyzed by nitric oxide (NO) production, the expression of inducible NO synthase (iNOS) and the mRNA level of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6). Nuclear translocation of nuclear factor-kappa B (NF-κB) p65 subunit and degradation of inhibitory kappa B (IκB) were examined by western blot. RESULTS: SSME inhibited LPS-induced NO production and iNOS expression without cytotoxicity. Up-regulation of LPS-induced pro-inflammatory cytokines, IL-1β and IL-6 was dose dependently attenuated by SSME. Exposure of pyrrolidine dithiocarbamate, an NF-κB specific inhibitor, accelerated the inhibitory effects of SSME on NO production and iNOS expression in LPS-stimulated cells. Moreover, translocation of NF-κB from the cytosol to the nucleus and degradation of IκB were decreased by treatment with SSME in LPS-induced cells. CONCLUSION: These results suggest that the SSME might have the inhibitory effects on inflammation, partly through inhibition of the NF-κB signaling pathway.
