In Vivo Validation Model of a Novel Anti-Inflammatory Scaffold in Interleukin-10 Knockout Mouse.
10.1007/s13770-018-0120-3
- Author:
Jung Yeon KIM
1
;
So Young CHUN
;
Sang Hoon LEE
;
Eugene LIH
;
Jeongshik KIM
;
Dae Hwan KIM
;
Yun Sok HA
;
Jae Wook CHUNG
;
Jun Nyung LEE
;
Bum Soo KIM
;
Hyun Tae KIM
;
Eun Sang YOO
;
Dong Keun HAN
;
Tae Gyun KWON
;
Byung Ik JANG
Author Information
1. Biomedical Research Institute, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu 41944, South Korea.
- Publication Type:Original Article
- Keywords:
IL-10 KO mice;
Anti-inflammatory scaffold;
Mg(OH)₂;
Renal regeneration;
Fibrosis
- MeSH:
Animals;
Ectodysplasins;
Fibrosis;
Gene Expression;
Immunohistochemistry;
Interleukin-10*;
Kidney;
Mice;
Mice, Knockout*;
Models, Animal;
Regeneration
- From:
Tissue Engineering and Regenerative Medicine
2018;15(4):381-392
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: We fabricated anti-inflammatory scaffold using Mg(OH)2-incorporated polylactic acid-polyglycolic acid copolymer (MH-PLGA). To demonstrate the anti-inflammatory effects of the MH-PLGA scaffold, an animal model should be sensitive to inflammatory responses. The interleukin-10 knockout (IL-10 KO) mouse is a widely used bowel disease model for evaluating inflammatory responses, however, few studies have evaluated this mouse for the anti-inflammatory scaffold. METHODS: To compare the sensitivity of the inflammatory reaction, the PLGA scaffold was implanted into IL-10 KO and C57BL/6 mouse kidneys. Morphology, histology, immunohistochemistry, and gene expression analyses were carried out at weeks 1, 4, 8, and 12. The anti-inflammatory effect and renal regeneration potency of the MH-PLGA scaffold was also compared to those of PLGA in IL-10 KO mice. RESULTS: The PLGA scaffold-implanted IL-10 KO mice showed kidneys relatively shrunken by fibrosis, significantly increased inflammatory cell infiltration, high levels of acidic debris residue, more frequent CD8-, C-reactive protein-, and ectodysplasin A-positive cells, and higher expression of pro-inflammatory and fibrotic factors compared to the control group. The MH-PLGA scaffold group showed lower expression of pro-inflammatory and fibrotic factors, low immune cell infiltration, and significantly higher expression of anti-inflammatory factors and renal differentiation related genes compared to the PLGA scaffold group. CONCLUSION: These results indicate that the MH-PLGA scaffold had anti-inflammatory effects and high renal regeneration potency. Therefore, IL-10 KO mice are a suitable animal model for in vivo validation of novel anti-inflammatory scaffolds.
