Profiling of Serum Metabolites Using MALDI-TOF and Triple-TOF Mass Spectrometry to Develop a Screen for Ovarian Cancer.
- Author:
Jun Hwa LEE
1
;
Yun Hwan KIM
;
Kyung Hee KIM
;
Jae Youl CHO
;
Sang Myung WOO
;
Byong Chul YOO
;
Seung Cheol KIM
Author Information
- Publication Type:Original Article
- Keywords: Ovarian neoplasms; Low-mass ions; Serum metabolite; Hypoxanthine; Mass spectrometry
- MeSH: Biomarkers; Colorectal Neoplasms; Female; Humans; Hypoxanthine; Lysophosphatidylcholines; Mass Screening; Mass Spectrometry*; Ovarian Neoplasms*; Pancreatic Neoplasms; Sensitivity and Specificity
- From:Cancer Research and Treatment 2018;50(3):883-893
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: We sought to develop a matrix assisted laser desorption ionization-time of flight (MALDI-TOF)-based, ovarian cancer (OVC), low-mass-ion discriminant equation (LOME) and to evaluate a possible supportive role for triple-TOF mass analysis in identifying metabolic biomarkers. MATERIALS AND METHODS: A total of 114 serum samples from patients with OVC and benign ovarian tumors were subjected to MALDI-TOF analysis and a total of 137 serum samples from healthy female individuals and patients with OVC, colorectal cancer, hepatobiliary cancer, and pancreatic cancer were subjected to triple-TOF analysis. An OVC LOME was constructed by reference to the peak intensity ratios of discriminatory low-mass ion (LMI) pairs. Triple-TOF analysiswas used to select and identify metabolic biomarkers for OVC screening. RESULTS: Three OVC LOMEs were finally constructed using discriminatory LMI pairs (137.1690 and 84.4119 m/z; 496.5022 and 709.7642 m/z; and 524.5614 and 709.7642 m/z); all afforded accuracies of > 90%. The LMIs at 496.5022 m/z and 524.5614 m/z were those of lysophosphatidylcholine (LPC) 16:0 and LPC 18:0. Triple-TOF analysis selected seven discriminative LMIs; each LMI had a specificity > 90%. Of the seven LMIs, fourwith a 137.0455 m/z ion atretention times of 2.04-3.14 minuteswere upregulated in sera from OVC patients; the ion was identified as that derived from hypoxanthine. CONCLUSION: MALDI-TOF–based OVC LOMEs combined with triple-TOF–based OVC metabolic biomarkers allow reliable OVC screening; the techniques are mutually complementary both quantitatively and qualitatively.
