The Prognostic Value of CD8⁺ and CD45RO⁺ T Cells Infiltration and Beclin1 Expression Levels for Early Postoperative Cholangitis of Biliary Atresia Patients after Kasai Operation.
10.3346/jkms.2018.33.e198
- Author:
Hong JIANG
1
;
Pengfei GAO
;
Huadong CHEN
;
Zhihai ZHONG
;
Man SHU
;
Zhichong ZHANG
;
Jinbiao SHE
;
Juncheng LIU
Author Information
1. Department of Pediatric Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. 1446447201@qq.com
- Publication Type:Original Article
- Keywords:
Biliary Atresia;
Kasai Portoenterostomy;
Postoperative Cholangitis;
CD45RO⁺ T Infiltration;
Beclin1
- MeSH:
Biliary Atresia*;
Cholangitis*;
Humans;
Liver;
Logistic Models;
ROC Curve;
T-Lymphocytes*
- From:Journal of Korean Medical Science
2018;33(30):e198-
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Postoperative cholangitis is a common but severe complication after Kasai portoenterostomy for biliary atresia (BA). This study aimed to identify its prognostic factors. METHODS: Two sets of liver paraffin-embedded tissue samples were collected from BA patients who received Kasai portoenterostomy (n = 25 and n = 31, respectively). Patients were divided into non-cholangitis and cholangitis groups. The infiltration of CD4+, CD8+, CD45RO+, CD68+ cells and expression of Beclin1 were quantitatively evaluated in immunohistochemical analysis. RESULTS: Cholangitis group had a significantly lower CD8+ T cell infiltration but a higher CD45RO+ cell infiltration, and a lower Beclin1 level than non-cholangitis group (all P < 0.01). Multivariate logistic regression analysis indicated that infiltration of CD8+ cells (odds ratio [OR], 0.112; 95% confidence interval [CI], 0.022–0.577) and CD45RO+ cells (OR, 3.88; 95% CI, 1.37–11.03), and Beclin1 level (OR, 0.088; 95% CI, 0.018–0.452) were independent influence factors for early postoperative cholangitis. Receiver operating characteristic (ROC) analysis showed that area under ROC curve (AUROC) values for CD8+ cells, CD45RO+ cells and Beclin1 were 0.857, 0.738 and 0.900, respectively. CONCLUSION: Our findings demonstrated the CD8+ cells, CD45RO+ cells and Beclin1 level possessed the prognostic value for early postoperative cholangitis following Kasai operation, which may be helpful to develop new prevention and treatment strategies for postoperative cholangitis.
