Phenotypic and Genomic Characterization of AmpC-Producing Klebsiella pneumoniae From Korea.
10.3343/alm.2018.38.4.367
- Author:
Mattia PALMIERI
1
;
Stephane SCHICKLIN
;
Andreu Coello PELEGRIN
;
Sonia CHATELLIER
;
Christine FRANCESCHI
;
Caroline MIRANDE
;
Yeon Joon PARK
;
Alex VAN BELKUM
Author Information
1. bioMérieux, Data Analytics Unit, La Balme Les Grottes, France. alex.vanbelkum@biomerieux.com
- Publication Type:Brief Communication
- Keywords:
AmpC;
Antibiotic resistance;
Whole Genome Sequencing;
Klebsiella pneumoniae;
Korea
- MeSH:
Drug Resistance, Microbial;
Genome;
Gram-Negative Bacteria;
Klebsiella pneumoniae*;
Klebsiella*;
Korea*;
Phenotype;
Polymerase Chain Reaction;
Prevalence
- From:Annals of Laboratory Medicine
2018;38(4):367-370
- CountryRepublic of Korea
- Language:English
-
Abstract:
The prevalence of multidrug-resistant gram-negative bacteria has continuously increased over the past few years; bacterial strains producing AmpC β-lactamases and/or extended-spectrum β-lactamases (ESBLs) are of particular concern. We combined high-resolution whole genome sequencing and phenotypic data to elucidate the mechanisms of resistance to cephamycin and β-lactamase in Korean Klebsiella pneumoniae strains, in which no AmpC-encoding genes were detected by PCR. We identified several genes that alone or in combination can potentially explain the resistance phenotype. We showed that different mechanisms could explain the resistance phenotype, emphasizing the limitations of the PCR and the importance of distinguishing closely-related gene variants.
