Endothelium Independent Effect of Pelargonidin on Vasoconstriction in Rat Aorta.
10.4062/biomolther.2017.197
- Author:
Young Sil MIN
1
;
Hyuk Jun YOON
;
Hyun Dong JE
;
Jong Hyuk LEE
;
Seong Su YOO
;
Hyun Sub SHIM
;
Hak Yeong LEE
;
Hyen Oh LA
;
Uy Dong SOHN
Author Information
1. Department of Medical Plant Science, College of Scienceand Engineering, Jung Won University, Goesan 28024, Republic of Korea.
- Publication Type:Original Article
- Keywords:
ERK1/2;
Fluoride;
MYPT1;
Pelargonidin;
Phorbol ester;
Rho-kinase
- MeSH:
Animals;
Anthocyanins;
Aorta*;
Blotting, Western;
Endothelium*;
Fluorides;
Fruit;
Humans;
Isometric Contraction;
Male;
Muscle, Smooth, Vascular;
Muscles;
Myosin-Light-Chain Phosphatase;
Phosphorylation;
Phosphotransferases;
Protein Kinases;
Rats*;
Relaxation;
rho-Associated Kinases;
Vasoconstriction*;
Vegetables
- From:Biomolecules & Therapeutics
2018;26(4):374-379
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, we investigated the effects of pelargonidin, an anthocyanidin found in many fruits and vegetables, on endothelium-independent vascular contractility to determine the underlying mechanism of relaxation. Isometric contractions of denuded aortic muscles from male rats were recorded, and the data were combined with those obtained in western blot analysis. Pelargonidin significantly inhibited fluoride-, thromboxane A2-, and phorbol ester-induced vascular contractions, regardless of the presence or absence of endothelium, suggesting a direct effect of the compound on vascular smooth muscles via a different pathway. Pelargonidin significantly inhibited the fluoride-dependent increase in the level of myosin phosphatase target subunit 1 (MYPT1) phosphorylation at Thr-855 and the phorbol 12,13-dibutyrate-dependent increase in the level of extracellular signal-regulated kinase (ERK) 1/2 phosphorylation at Thr202/Tyr204, suggesting the inhibition of Rho-kinase and mitogen-activated protein kinase kinase (MEK) activities and subsequent phosphorylation of MYPT1 and ERK1/2. These results suggest that the relaxation effect of pelargonidin on agonist-dependent vascular contractions includes inhibition of Rho-kinase and MEK activities, independent of the endothelial function.
