Evaluation of prooxidant-antioxidant balance in in vitro fertilization-conceived mice.
10.5653/cerm.2018.45.2.82
- Author:
Hamid AHMADI
1
;
Fardin FATHI
;
Ashraf MOEINI
;
Fardin AMIDI
;
Aligholi SOBHANI
Author Information
1. Department of Anatomy, Medical School, Tehran University of Medical Sciences, Tehran, Iran. sobhania@tums.ac.ir
- Publication Type:Original Article
- Keywords:
In vitro fertilization;
Mice;
Offsprings;
Oxidative stress;
Prooxidant-antioxidant balance
- MeSH:
Animals;
Antioxidants;
Asthma;
Fertilization in Vitro;
Free Radicals;
Hypersensitivity;
In Vitro Techniques*;
Incidence;
Litter Size;
Mice*;
Oxidative Stress;
Reproductive Techniques, Assisted
- From:Clinical and Experimental Reproductive Medicine
2018;45(2):82-87
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Concerns about the safety of assisted reproductive technology (ART) have been raised, as some studies have shown elevated incidence rates of childhood cancer, asthma, allergies, and other diseases in ART-conceived babies. Findings regarding the health of ART-conceived babies are controversial. The present study was conducted to evaluate the prooxidant-antioxidant balance (PAB) in in vitro fertilization (IVF)-conceived mice in comparison to naturally conceived offspring. METHODS: Mice (6–8 weeks) were divided into two groups (IVF-conceived and naturally conceived) matched by sex, age, weight, and litter size. A 1-mL blood sample was taken and the sera were separated. The oxidant-antioxidant balance was evaluated using a fast and reliable PAB assay. The results were expressed as mean±standard deviation. RESULTS: The mean PAB values (HK units) in the IVF-conceived and naturally conceived groups were 59.70±22.30 and 54.70±18.22, respectively (p=0.82). CONCLUSION: Since free radicals contribute to several pathological conditions and antioxidants play an important protective role against oxidative stress, evaluating the oxidant-antioxidant balance is very important. Although the results of this study showed that the quality of the defense mechanism against free radicals was not significantly different between the IVF-conceived and naturally conceived mice, other parameters of metabolic dysfunction need to be measured.
