Two combined amino acids promote sleep activity in caffeine-induced sleepless model systems.
10.4162/nrp.2018.12.3.208
- Author:
Ki Bae HONG
1
;
Yooheon PARK
;
Hyung Joo SUH
Author Information
1. Department of Biological Sciences and Environmental Sciences Program, Southern Illinois University-Edwardsville, Edwardsville, IL 62026, United States.
- Publication Type:Original Article
- Keywords:
Sleep;
caffeine;
GABA;
5-HTP;
insomnia
- MeSH:
5-Hydroxytryptophan;
Amino Acids*;
Animals;
Caffeine;
Diptera;
Drosophila melanogaster;
Electroencephalography;
Fruit;
gamma-Aminobutyric Acid;
Humans;
Mice;
Mice, Inbred ICR;
Motor Activity;
Rats, Sprague-Dawley;
Sleep Initiation and Maintenance Disorders;
Vertebrates
- From:Nutrition Research and Practice
2018;12(3):208-214
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVES: The aim of this study was to evaluate the biological and sleep-promoting effects of combined γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) using caffeine-induced sleepless fruit flies, ICR mice, and Sprague-Dawley rats. MATERIALS/METHODS: Video-tracking analysis was applied to investigate behavioral changes of Drosophila melanogaster. Pentobarbital-induced sleep test and electroencephalogram (EEG) patterns were used for analysis of sleep latency, duration, and quantity and quality of sleep in vertebrate models. RESULTS: Administration of combined GABA/5-HTP could significantly reverse the caffeine induced total distance of flies (P < 0.001). Also, individually administered and combined GABA/5-HTP significantly increased the total sleeping time in the caffeine-induced sleepless ICR mice (P < 0.001). In the caffeine-induced sleepless SD-rats, combined GABA/5-HTP showed significant differences in sleep quality between individual amino acid administrations (P < 0.05). CONCLUSIONS: Taken together, we identified inhibitory effects of combined GABA/5-HTP in locomotor activity, sleep quantity and quality in caffeine-induced sleepless models, indicating that combined GABA/5-HTP may be effective in patients with insomnia by providing sufficient sleep.
